alexa Development of Stability Indicating Densitometric and Enhanced sensitivity Spectrofluorimetric Methods for Determination of Zaleplon in Presence of its Acidic Degradation Products | Abstract
ISSN : 2153-2435

Pharmaceutica Analytica Acta
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Research Article

Development of Stability Indicating Densitometric and Enhanced sensitivity Spectrofluorimetric Methods for Determination of Zaleplon in Presence of its Acidic Degradation Products

Sawsan A Abdel Razeq1, Suzan M Soliman2 and Amal S Mohamed2*

1Analytical Chemistry Department,Faculty of Pharmacy (Girls),Al-Azhar University,Cairo,Egypt

2National Organization for Drug Control and Research (NODCAR). 6 Abu Hazem Street,Pyramids Ave. P. O. Box 29.Giza,Egypt

*Corresponding Author:
Amal S Mohamed
National Organization for Drug Control and Research (NODCAR)
6 Abu Hazem Street, Pyramids Ave. P. O. Box 29.Giza, Egypt
E-mail: [email protected]

Received date: May 31, 2013; Accepted date: June 26, 2013; Published date: June 28, 2013

Citation: Abdel Razeq SA, Soliman SM, Mohamed AS (2013) Development of Stability Indicating Densitometric and Enhanced Sensitivity Spectrofluorimetric Methods for Determination of Zaleplon in Presence of its Acidic Degradation Products. Pharm Anal Acta 4:256. doi: 10.4172/2153-2435.1000256

Copyright: © 2013 Abdel Razeq SA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Zaleplon is easily degradable into its acidic degradation products, so two stability- indicating methods were developed for determination of zaleplon in the presence of these degradation products and are successfully applied to quantify zaleplon in pharmaceutical preparations. The first was a densitometric evaluation of thin-layer chromatograms of the drug using a mobile phase of ethyl acetate – ammonia (33%) - methanol (8.5:0.5:1, v/v/v). The chromatograms were scanned at 338 nm, a wavelength at which zaleplon can be readily separated from its degradation products and determined in the range of 0.5-2.5 μg/spot with mean percentage recovery of 100.79 ± 0.65%. The second method was based on measuring the fluorescence intensity of zaleplon at λex/λem =350 nm/460 nm. The effect of micelle medium on the fluorescence emission was studied which revealed that the anionic surfactant of sodium lauryl sulphate has strong sensitizing effect for the fluorescence. The fluorescence intensity plot was linear over the range of 0.1-3.6 μg/ml with mean percentage recovery of 100.39 ± 1.12%. Determination was also successful when analyzing zaleplon in a formulation in the form of Siesta capsules. Results were statistically analyzed and found to be in accordance with those given by a reported method.

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