alexa Development of Surface Plasmon Resonance (SPR) Based Immuno-Sensing System for Detection of Fungal Teliospores of Karnal Bunt (Tilletia indica), a Quarantined Disease of Wheat
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Development of Surface Plasmon Resonance (SPR) Based Immuno-Sensing System for Detection of Fungal Teliospores of Karnal Bunt (Tilletia indica), a Quarantined Disease of Wheat

Sadhna Singh1, Manoj Singh1, Gohar Taj1, Sanjay Gupta2 and Anil Kumar1*

1Department of Molecular Biology & Genetic Engineering, College of Basic Sciences & Humanities, G. B. Pant University of Agriculture & Technology, Pantnagar, Uttaranchal, India

2Department of Biotechnology, SBS PG Institute of Biomedical Sciences, Balawala, Dehradun, Uttarakhand, India

*Corresponding Author:
Dr. Anil Kumar, Professor & Head
Department of Molecular Biology & Genetic Engineering
College of Basic Sciences & Humanities
G.B. Pant University of Agriculture & Technology
Pantnagar-263 145, U.S. Nagar
Uttaranchal, India
E-mail: [email protected]

Received Date: September 05, 2012; Accepted Date: October 15, 2012; Published Date: October 17, 2012

Citation: Singh S, Singh M, Taj G, Gupta S, Kumar A (2012) Development of Surface Plasmon Resonance (SPR) Based Immuno-Sensing System for Detection of Fungal Teliospores of Karnal Bunt (Tilletia Indica), a Quarantined Disease of Wheat. J Biosens Bioelectron 3:125. doi: 10.4172/2155-6210.1000125

Copyright: © 2012 Singh S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

This paper first time describes Surface Plasmon Resonance (SPR) based immuno-sensing system for detection of fungal teliospores of Karnal Bunt (KB) disease of wheat incited by Tilletia indica. The results of present study were compared with the results of lab scale ELISA developed earlier in our lab. The approach involves the use of a rabbit polyclonal (anti-teliospore) antibody and a SPR sensor for label-free detection of teliospores from the model organism Tilletia indica. When the interaction between binder (anti-teliospore antibody) and teliosporic antigen(s) as analyte occurs at the sensor surface, a sensorgram was obtained in real time by plotting the signal against time. Under optimised assay conditions as few as 5 teliospores were detected by ELISA which justifies the greater affinity of raised anti-teliospore antibodies for its antigen. The sensitivity of each immunosensor constructed with
the different amounts of antigen solution is determined by sensogram analysis showed detection sensitivity as low as 625 pg equivalent to 2.5 teliospores over the sensor surface by getting an angle of dip indicating greater affinity of raised anti-teliospore antibodies. Thus, SPR based affinity sensor showed higher sensitivity due to greater signal response. The results of cross reactivity studies using different related fungal spores/teliospores showed almost differential SPR response indicating cross reaction using immunosensing system. The cross-reactivity of anti-teliospore antibodies with related fungal spores/teliospores can be explained due to its polyclonal nature and also due to antigenic homology amongst pathogens used in present study.

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
In Association with
 
romanian
 
icamb2015
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_p[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords