alexa Diagnostic Aspects and Novel Approach for Treatment of
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Diagnostic Aspects and Novel Approach for Treatment of Antibiotic-Resistant Bacteria Isolated from Diarrheal Calves using Silver, Gold and Copper Nanoparticles

Ayman El Behiry1,2*

1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Sadat City University, Egypt

2Department of Public Health, Microbiology, College of Public Health, Qassim University, Kingdom of Saudi Arabia (KSA)

*Corresponding Author:
El Behiry A
Department of Bacteriology, Mycology and Immunology
Faculty of Veterinary Medicine, Sadat City University, Egypt
Tel: 00966532207969
E-mail: [email protected]

Received date: July 12, 2014; Accepted date: July 31 2014; Published date: August 02, 2014

Citation: El Behiry A (2014) Diagnostic Aspects and Novel Approach for Treatment of Antibiotic-Resistant Bacteria Isolated from Diarrheal Calves using Silver, Gold and Copper Nanoparticles. J Bacteriol Parasitol 5:195. doi: 10.4172/2155-9597.1000195

Copyright: © 2014 El Behiry A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

This study was performed to identify various bacteria from feces of calves suffering from diarrhea, and to determine in vitro antimicrobial activity of silver, gold and copper nanoparticles against the antibiotic-resistant bacteria isolated from fecal samples. Fecal samples were collected from 153 diarrheic calves and primarily tested for the presence of Escherichia coli, Salmonella spp. and Staphylococcus aureus using bacteriological examination, biochemical reactions and polymerase chain reaction. The minimum inhibitory concentrations of silver, gold and copper nanoparticles against identified bacterial isolates were determined by a broth dilution method. 84 bacterial isolates from the 153 fecal samples were identified using bacteriological, biochemical and genotypical methods. Escherichia coli was considered the most frequent bacterium isolated numbering 31 (36.90%) followed by Salmonella sp. as the second most prevalent 16 (19.04%). Further isolates such as Staphylococcus aureus 10 (11.90%) were isolated and identified. The MIC values of silver, gold and copper nanoparticles with a size of 10 nanometers (nm) against the three types of bacteria were ranged from 0.625 to 10 μg/ml, 2.5 to 20 μg/ml and 2.5 to 20 μg/ml, respectively. While these values with a size of 20 nm were 0.312 to 2.5 μg/ml, 1.25 to 10 μg/ml and 2.5 to 10 μg/ml, respectively. Additionally, the mean time of the antimicrobial action of silver, gold and copper nanoparticles at concentration 10 nm against all isolates was 5 min, 30 min and 15 min, respectively. Using of these nanoparticles in a concentration of 20 nm, the mean time was 1, 15 and 5 min, respectively. These in vitro results clearly indicate that the silver, gold and copper nanoparticles might have a superior activity and rapid onset of action against the Gram negative bacteria E. coli and Salmonella spp. and the Gram positive bacteria Staphylococcus aureus with diarrheal origin.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords