alexa Dicer Silencing by siRNA in Cerebellar Granule Neurons | OMICS International| Abstract
ISSN: 2157-7099

Journal of Cytology & Histology
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  • Research Article   
  • J Cytol Histol 2019, Vol 10(2)
  • DOI: 10.4172/2157-7099.1000539

Dicer Silencing by siRNA in Cerebellar Granule Neurons

Christian Barbato*
Department of Sense Organs, Institute of Cell Biology and Neurobiology (IBCN), National Research Council (CNR), University Sapienza of Rome, Viale del Policlinico, 155, 00161 Rome, Italy
*Corresponding Author : Christian Barbato, Department of Sense Organs, Institute of Cell Biology and Neurobiology (IBCN), National Research Council (CNR), University Sapienza of Rome, Viale Del Policlinico, 155, 00161 Rome, Italy, Tel: +39-0649976741, Email: [email protected]

Received Date: Apr 17, 2019 / Accepted Date: May 08, 2019 / Published Date: May 14, 2019

Abstract

RNA-mediated gene silencing is a fundamental mechanism of gene expression regulation. A main component of RNA silencing machinery, the RNase III Dicer enzyme, catalyzes the processing of double-stranded RNAs (dsRNAs) into ≈21-25 nucleotide-long small interfering (si)RNAs and micro (mi)RNAs, an essential step in the biogenesis of small non-coding RNA molecules. In previous report was reported the Dicer localizes with ER and Golgi network in post-mitotic neurons, increasing its expression during the development and maturation in cultured neurons. With the aim to study the components of RNA-induced silencing complex (RISC), it was investigated the effect of Dicer silencing on survival in cultured cerebellar granule neurons (CGNs). In this immnofluorescence study was observed that Dicer silencing by siRNA facilitates the apoptotic neuronal cell death in CGNs

Keywords: RNA induced silencing complex; Dicer; RNAi; Posttranscriptional gene regulation; Post-mitotic neurons; Apoptosis

Citation: Barbato C (2019) Dicer Silencing by siRNA in Cerebellar Granule Neurons. J Cytol Histol 10: 539. Doi: 10.4172/2157-7099.1000539

Copyright: © 2019 Barbato C. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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