alexa Differential Effects of CBZ-Induced Catalysis and Cytochrome Gene Expression in Three Dimensional Zebrafish Liver Cell Culture | OMICS International | Abstract
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
Open Access

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Research Article

Differential Effects of CBZ-Induced Catalysis and Cytochrome Gene Expression in Three Dimensional Zebrafish Liver Cell Culture

Yoonah Jeong1, Chang-Beom Park1, In-Hyuk Baek1, Kwanghun Jeong1,3, Seungyun Baik1,2* and Young Jun Kim1,3*

1Environmental Safety Group, Korea Institute of Science and Technology Europe (KIST Europe), Forschungsgesellschaft mbH, Campus E7 1, Saarbruecken, Germany

2Department of Energy and Environmental Engineering, University of Science and Technology, Daejeon, Korea

3Department of Biomedical Engineering, University of Science and Technology, Daejeon, Korea

*Corresponding Author:
Seungyun Baik
Environmental Safety Group
Korea Institute of Science and Technology Europe (KIST Europe)
Forschungsgesellschaft mbH, Campus E7 1
Saarbruecken, Germany
Tel: +4906819382327
E-mail: [email protected]
 
Young Jun Kim
Environmental Safety Group
Korea Institute of Science and Technology Europe (KIST Europe), Forschungsgesellschaft mbH
Campus E7 1, Saarbruecken, Germany
Tel: +4906819382327
E-mail: [email protected]

Received date: September 02, 2016; Accepted date: September 20, 2016; Published date: September 27, 2016

Citation: Jeong Y, Park CB, Baek IH, Jeong K, Baik S, et al. (2016) Differential Effects of CBZ-Induced Catalysis and Cytochrome Gene Expression in Three Dimensional Zebrafish Liver Cell Culture. J Environ Anal Toxicol 6:404. doi: 10.4172/2161-0525.1000404

Copyright: © 2016 Jeong Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Two dimensional (2D) cell cultures have been the standard in vitro model for environmental safety research. However, it does not properly mimic in vivo system which hampered cellular proliferation and differentiation over an extended culture time and quickly lost its function. In this study, we constructed a new three dimensional (3D) zebrafish liver cell model that more closely mimics the in vivo micro-environment for pharmaceuticals metabolism studies. The magnetic levitation method was used to maintain the controlled and uniform 3D spheroids. These spheroids were then tested with carbamazepine (CBZ) treatment to determine the applicability of this assay in metabolism study. The results demonstrated that the 3D cell model is more resistant to CBZ over 2D cell culture and in vivo and 3D culture cells are noticeable in CYP1a1 activity and the concentration of carbamazepine-10,11-epoxide (CBZ-EP) than 2D cell culture. This 3D cell culture model will provide useful information toward the development of improved biomimetic organ functions for alternatives to animal testing in environmental contaminants.

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