Differentiation of Embryonic Stem Cells and Oxidative DNA Damage / DNA Repair SystemsHirano T1* and Tamae K2
- Corresponding Author:
- Prof. Takeshi Hirano
Department of Life and Environment Engineering
Faculty of Environmental Engineering, The University of Kitakyushu
1-1 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka, 808-0135, Japan
E-Mail: [email protected]
Received Date: April 08, 2012; Accepted Date: May 05, 2012; Published Date: May 07, 2012
Citation: Hirano T, Tamae K (2012) Differentiation of Embryonic Stem Cells and Oxidative DNA Damage / DNA Repair Systems. J Stem Cell Res Ther S10:005. doi:10.4172/2157-7633.S10-005
Copyright: © 2012 Hirano T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
To maintain genomic integrity, cells are equipped with a defense capacity against DNA damage generated in genomic DNA. The avoidance of mutations in genomic DNA is especially critical for undifferentiated cells, such as Embryonic stem (ES) cells. Among the many factors damaging DNA, Reactive Oxidative Species (ROS) are crucial and unavoidable, and thus the roles of ROS in cell differentiation and cellular functions have been extensively studied. However, the biological significance of ROS generation in cell differentiation remains a matter of debate. Among the types of DNA damage due to ROS, 8-oxoguanine (8-oxo-Gua) has been well studied and is known to generate GC-to-TA point mutations in genomic DNA. We recently analyzed the differences in the resistance to 8-oxo-Gua generation between undifferentiated cells and their differentiated counterparts. Our studies indicated that undifferentiated cells were more resistant to 8-oxo-Gua generation, in comparison to differentiated cells. In this short review, we describe the relationship between ES cell differentiation and oxidative DNA damage / DNA repair systems, by summarizing our previous work and that of other researchers.