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Diminished IL-10 production is Associated with Impaired Versatility of Monocytes in Familial Mediterranean Fever | OMICS International | Abstract
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
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Research Article

Diminished IL-10 production is Associated with Impaired Versatility of Monocytes in Familial Mediterranean Fever

Tigran K. Davtyan1*, Gagik S. Hakobyan2, Samvel A. Avetisyan3, Anna G. Sukiasyan4 and Yuri T. Aleksanyan4
1Laboratory of Immunology and Virology, "Armenicum" Research Centre, CJSC Armenicum, 37 Nalbandyan St., Yerevan, Republic of Armenia
2Department of Internal Medicine, Yerevan State Medical University, Koryun 2 St., Yerevan, Republic of Armenia
3Department of Pathophysiology; Yerevan State Medical University, Koryun 2 St., Yerevan, Republic of Armenia
4Laboratory of Epidemiology and Immunology, Institute of Epidemiology, Virology and Medical Parasitology, Ministry of Health RA, Yerevan, Armenia
Corresponding Author : Dr. Tigran K. Davtyan, PhD, ScD
Analytical Laboratory Branch
Scientific Centre of Drug and Medical Technology Expertise JSC, Armenia
Tel: +374 10 23-72-61
Fax: +374 10 28-07-33
E-mail: [email protected]
Received: January 15, 2014; Accepted: March 10, 2014; Published: March 17, 2014
Citation: Davtyan TK, Hakobyan GS, Avetisyan SA, Sukiasyan AG, Aleksanyan YT (2014) Diminished IL-10 production is Associated with Impaired Versatility of Monocytes in Familial Mediterranean Fever. J Clin Cell Immunol 5:196. doi:10.4172/2155-9899.1000196
Copyright: © 2014 Davtyan TK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Purpose: The nature of the heightened endotoxin sensitivity state observed in Familial Mediterranean Fever (FMF) at present remains unknown. To assess the possibility that IL-10 plays a role in setting the inflammatory threshold, we studied IL-10 production by monocytes and dendritic cells as well as endotoxin tolerance induction in FMF patients.
Methods: 46 attack-free FMF patients were included in this study. The production of IL-10 by NLR- or TLRagonist- stimulated monocytes and dendritic cells were assayed either by conventional ELISA or flow cytometry. The versatility of monocytes was studied by measuring the production of IL-10 and IL-1β after stimulation by pro- and anti-inflammatory agents, and after stimulation arrest or a further counter stimulation. Monocyte endotoxin tolerance and cross-tolerance induction were assayed by measuring the production of IL-1β, IL-10, TNF-α and IFN-γ after prestimulation by NLR- or TLR-ligands and after re-stimulation with LPS.
Results: In FMF patients, we observed down-regulation of circulating CD36+ peripheral blood lymphoid cells but not monocytes, constitutively producing IL-10. The production of IL-10 by TLR- and NLR-agonist-stimulated monocytes and dendritic cells declines in FMF patients. Monocytes isolated from FMF patients failed to switch from a pro-inflammatory activated state to anti-inflammatory phenotype and still produce IL-1β but not IL-10, which cause impaired endotoxin tolerance and cross-tolerance induction. The IL-10 production and endotoxin tolerance induction by monocytes and dendritic cells were restored by NOD2- ligand MDP and colchicine treatment.
Conclusion: The reduced IL-10 production was associated with the impaired setting of feedback inhibition of inflammatory response and caused impaired resolution of inflammation and endotoxin tolerance induction.

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