Diospyros cuneata Inhibition of Fusarium oxysporum: Aqueous Extract and its Encapsulation by Ionic Gelation
- *Corresponding Author:
- Rodríguez-García CM
Unidad de Biotecnología
Centro de Investigación Científica de Yucatán, A.C.
Calle 43 No. 130 Chuburná de Hidalgo
CP 97200 Mérida, Yucatán, México
E-mail: [email protected]
Received date: January 07, 2016; Accepted date: January 25, 2016; Published date: January 28, 2016
Citation: Ruiz-Ruiz JC, Peraza-Echeverría L, Soto-Hernández RM, San Miguel-Chávez R, Pérez-Brito D, et al. (2016) Diospyros cuneata Inhibition of Fusarium oxysporum: Aqueous Extract and its Encapsulation by Ionic Gelation. J Plant Pathol Microbiol 7:332. doi:10.4172/2157-7471.1000332
Copyright: © 2016 Ruiz-Ruiz JC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The application of plant extracts to control fungal crop pathogens is an ecological strategy that could potentially be useful in agriculture. Aqueous extracts of some species of the genus Diospyros spp have been tested against fungal pathogens of crops. Nevertheless, there is no information about the inhibitory effect of aqueous extracts of Diospyros cuneata on the micelial growth of Fusarium oxysporum, a pathogen responsible for “Panama disease” and “vanilla stem rot disease”. Therefore, the antifungal activity of aqueous extracts of Diospyros cuneata leaves collected during the dry and rainy seasons was tested in-vitro against spores of Fusarium oxysporum. Only the aqueous extract from leaves collected during the dry season had an inhibitory effect on the micelial growth of asexual spores (2.5% minimum inhibitory concentration). Phytochemical analysis showed that both aqueous extracts contained mainly flavonoids and tannins; the chromatographic profile showed a larger abundance of polar compounds in the dry season extract. Furthermore, the antifungal activity observed is probably correlated with the abundance of some secondary metabolites produced by water stress and dry season conditions. Thebioactivityof aqueous extracts of Diospyros cuneata leaves could be stored and released through encapsulation; an effective example of this was tested using alginate-inulin to prepare microbeads by ionic gelation.