Direct Colony Nested-PCR for the Detection of Fusarium oxysporum f. sp. Psidii Causing Wilt Disease in Psidium guajava L.
|Rupesh Kumar Mishra1,2*, Dev Kumar Verma3, BK Pandey2, Neelam Pathak1 and Mohd Zeeshan1|
|1Department of Biosciences, Integral University, Lucknow, India|
|2Molecular Diagnostic Laboratory, Division of Crop Protection, Central Institute for Subtropical Horticulture, Lucknow, India|
|3National Bureau of Fish Genetic Resources, Lucknow, India|
|Corresponding Author :||Rupesh Kumar Mishra
Department of Biosciences
Integral University, Lucknow, India
|Received March 25, 2014; Accepted May 14, 2014; Published May 16, 2014|
|Citation: Mishra RK, Verma DK, Pandey BK, Pathak N and Zeeshan M (2014) Direct Colony Nested-PCR for the Detection of Fusarium oxysporum f. sp. Psidii Causing Wilt Disease in Psidium guajava L.. J Horticulture 1:105. doi:10.4172/2376-0354.1000105|
|Copyright: © 2014 Mishra RK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Guava (Psidium guajava L.) is one of the important fruit crops which are grown in tropical and subtropical countries including India. Wilt is an important disease in guava which is caused by Fusarium oxysporum f. sp. psidii (Fop) as a major obstacle for guava fruit production. It is manifested symptomatically with alterations in the development process such as premature shedding of leaves, pre-maturation of fruits, entire/whole tree defoliation and eventually death of the plant. In this study a colony PCR assay was developed for direct amplification of Internal Transcribed Spacer (ITS) region of fungal rDNA of Fop isolates. For this, two sets of primers were developed for amplification of ITS region. . The sensitivity of both the primer pair was ranged up to 10-6 and 10-7 dilutions of the pure mycelium suspension. This study provides new insight for rapid, sensitive and specific molecular detection of Fop isolates, and also useful for earlier diagnostic for better management of guava wilt disease.