DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM LEAF AND STEM EXPLANTS OF NOTHAPODYTES FOETIDA: A CRITICALLY ENDANGERED PLANT SPECIES
|Suresh Khadke and Aniket Kuvalekar*
Nutrigenomics and Functional Foods Laboratory, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth Deemed University, Pune-Satara Road, Katraj, Pune- 411043, India
|Corresponding Author: Aniket Kuvalekar, Nutrigenomics and Functional Foods Laboratory, Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth Deemed University, Pune-Satara Road, Katraj, Pune- 411043, India|
|Received: 17 January 2013 Accepted: 27 January 2013|
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An efficient protocol for the regeneration of ‘critically endangered’ Nothapodytes foetida plants via direct somatic embryogenesis was developed. Somatic embryos were induced from leaf and stem explants cultured on Murashige and Skoog medium supplemented with 0.5-3.0 mg 1-1 thidiazuron and 20% coconut water. Histological examination revealed that somatic embryos were induced directly from the explants. Maturation of somatic embryos was accomplished with same induction medium. Germination and regeneration efficiency of somatic embryos was found to be 90.3±3.20. The regenerated plants were morphologically similar to seed-derived plants. This system may prove to be beneficial for ex-situ conservation as well as for amenability of this endangered plant for genetic manipulation. This is the first report of direct somatic embryogenesis in Nothapodytes foetida with significantly high plantlet regeneration frequency.