alexa Discovery of Novel Proteins form Injured Rat Pancreatic Extract using MALDI-TOF/MS-based Proteomics
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
Open Access

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Research Article

Discovery of Novel Proteins form Injured Rat Pancreatic Extract using MALDI-TOF/MS-based Proteomics

Hongbin Xie1, Hui Zhang2, Hui Qi1, Yunshuai Wang1, Chun-Yan Deng1 and Fu-Rong Li1,3*

1The Key Laboratory of Stem Cell and Cellular Therapy, The Second Clinical Medical College (Shenzhen People’s Hospital), Jinan University, Shenzhen, China

2Laboratory of Cancer Cell Proteomics, Nevada Cancer Institute, Las Vegas, NV 89135, USA

3Shenzhen Institute of Gerontology, Shenzhen, China

*Corresponding Author:
Fu-Rong Li
The Key Laboratory of Stem Cell and Cellular Therapy
The Second Clinical Medical College (Shenzhen People’s Hospital)
Jinan University, No.1017 Dongmen North Road
Shenzhen 518020, China
Tel: +86755-25533018
Fax: +86755-25533497
E-mail: [email protected]

Received date: July 10, 2013; Accepted date: August 05, 2013; Published date: August 12, 2013

Citation: Xie H, Zhang H, Qi H, Wang Y, Deng CY, et al. (2013) Discovery of Novel Proteins form Injured Rat Pancreatic Extract using MALDI-TOF/MS-based Proteomics. J Proteomics Bioinform 6:158-163. doi:10.4172/jpb.1000275

Copyright: © 2013 Xie H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Injured pancreatic tissue extract contains transcription proteins that are considered as specific soluble proteins, which contribute in promoting the trans-differentiation of stem cells into insulin-producing cells (IPCs). In this present study, 60% of the pancreatic tissues of Sprague-Dawley (SD) rats were removed, and newborn and normal pancreatic tissues were removed after 48 h to extract tissue fluid. Two-dimensional gel electrophoresis (2-DE) separation and spot analysis were conducted on differentially expressed proteins, and peptides were obtained after enzymatic digestion. Twenty two-fold or above differentially expressed proteins were identified via matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF/MS), among which, five proteins were related to pancreatic development and differentiation. Moreover, the expression patterns of four proteins detected with Western blot analysis were in agreement with those detected via 2-DE. Our results and those of the bioinformatics analysis suggest that these novel proteins from injured rat pancreatic extract can be a potential source for stem cell differentiation into IPCs.

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