Discovery of Three Newly Described Single Nucleotide Polymorphisms in Mitochondrial DNA Hypervariable Region I (HVI) and Estimation of Variants and Haplotypes Encompassing Nucleotide Positions 16024- 16365
- *Corresponding Author:
- Imad H
Department of Molecular Biology
Putra University, Kuala Lumpur City, Malaysia
E-mail: [email protected]
Received date: November 05, 2013; Accepted date: December 16, 2013; Published date: December 19, 2013
Citation: Imad H, Abeer F, Cheah Y, Mohammed J, Aamera O (2013) Discovery of Three Newly Described Single Nucleotide Polymorphisms in Mitochondrial DNA Hypervariable Region I (HVI) and Estimation of Variants and Haplotypes Encompassing Nucleotide Positions 16024-16365. J Forensic Res 5:209. doi: 10.4172/2157-7145.1000209
Copyright: © 2013 Imad H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The aims of this research are to study the mitochondria noncoding region by using the Sanger sequencing technique and establish the degree of variation characteristic of a fragment. FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. A portion of a noncoding region encompassing positions 16024-16365 amplified in accordance with the Anderson reference sequence. PCR products purified by EZ-10 spin column then sequenced and detected by using the ABI 3730xL DNA Analyzer. The most frequent variant differed in the single position A16227G, according to the CRS sequence. A new polymorphic position 16046, 16105 and 16141 are described may in future be suitable sources for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence most promising for identification variants.