Diversity, Abundance and Distribution of O-linked Glycosylation Pathway Enzymes in Prokaryotes-A Comparative Genomics Study
Manjeet Kumar and Petety V. Balajia*
Department of Biosciences and Bioengineering Indian Institute of Technology Bombay, Powai, Mumbai 400 076, India
- *Corresponding Author:
- Dr. Petety V. Balaji
Department of Biosciences and Bioengineering
Indian Institute of Technology Bombay
Powai, Mumbai 400 076, India
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E-mail: [email protected]
Received Date: July 01, 2014; Accepted Date: July 22, 2014; Published Date: July 31, 2014
Citation: Kumar M, Balajia PV (2014) Diversity, Abundance and Distribution of O-linked Glycosylation Pathway Enzymes in Prokaryotes-A Comparative Genomics Study. J Glycomics Lipidomics 4:117. doi: 10.4172/2153-0637.1000117
Copyright: © 2014 Kumar M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In prokaryotes, the protein protein N- and O-glycosylation pathways (GlyPW) have been experimentally characterised
in some of the organisms. Identifying GlyPWs in other prokaryotes is essential to understand the role of glycosylation.
Herein we report a BLASTp and a hidden Markov model (HMM)-profile based comparative genomics approach to identify
putative O-glycosylation enzymes in completely sequenced prokaryotic genomes using the experimentally characterized
O-GlyPW enzymes as query sequences. Homologs for enzymes of all five categories viz., initiation, modification,
extension, flippase and oligosaccharyltransferase are found in 128 organisms and no homolog is found for any of these
in 52 organisms. A large number of organisms have homologs for all categories except oligosaccharyltransferases, which
show high sequence diversity. Thus, O-GlyPW enzyme homologs are widely prevalent. Most of the 128 organisms are
proteobacteria and more than half are pathogenic. The pattern of distribution of homologs indicates species- and strainspecific
variations and acquisition of homologs by horizontal gene transfer.