alexa Diversity and Molecular Characterization of Dominant Bacillus amyloliquefaciens (JNU-001) Endophytic Bacterial Strains Isolated from Native Neem Varieties of Sanganer Region of Rajasthan| Abstract

ISSN: 2376-0214

Journal of Biodiversity, Bioprospecting and Development

  • Research Article   
  • J Biodivers Biopros Dev 2014, Vol 1(1): 115
  • DOI: 10.4172/2376-0214.1000115

Diversity and Molecular Characterization of Dominant Bacillus amyloliquefaciens (JNU-001) Endophytic Bacterial Strains Isolated from Native Neem Varieties of Sanganer Region of Rajasthan

Kartikeya Tiwari* and Haresh Kumar Thakur
Microbial Biotechnology Laboratory, Jaipur National University, Jaipur, Rajasthan, India
*Corresponding Author : Kartikeya Tiwari, Microbial Biotechnology Laboratory, Jaipur National University, Jaipur, Rajasthan, India, Tel: 91-9983762112, Email: [email protected]

Received Date: Mar 17, 2014 / Accepted Date: Jun 02, 2014 / Published Date: Jul 05, 2014

Abstract

Higher plants are ubiquitously colonized with bacterial endophytes that often lack readily detectable structures. In the present study seven novel endophytic bacterial species viz; Bacillus amyloliquefaciens (JNU-001), Burkholderia denitrificans (JNU-002), Pseudomonas aeruginosa (JNU-003), Xanthomonas campestris (JNU-004), Azotobacter tropicalis (JNU-005), Acetobacter xylinum (JNU-006) and Azospirillum lipoferum (JNU-007) has been recovered from native neem varieties at Sanganer areas namely Khonagorion, Muhana, Jagatpura, Goner, Watika, Kalwara and Bagru of Rajasthan state. In which Bacillus amyloliquefaciens was found dominant and associated endophytically in all the plants. Molecular characterization of the isolates was performed using 16S r-DNA restriction analysis. Similarity index in unweighted pair group method with arithmetic mean programme clustered the isolates according to their geographical distribution and confirmed the endophytic association between these novel bacterial strains and neem varieties.

Keywords: Endophytes; Nutrient agar; UPGMA analysis; NTSYS; ARDRA

Citation: Tiwari K,Thakur HK (2014) Diversity and Molecular Characterization of Dominant Bacillus amyloliquefaciens (JNU-001) Endophytic Bacterial Strains Isolated from Native Neem Varieties of Sanganer Region of Rajasthan. J Biodivers Biopros Dev 1: 115. Doi: 10.4172/2376-0214.1000115

Copyright: ©2014 Tiwari K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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