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ISSN: 2329-9002

Journal of Phylogenetics & Evolutionary Biology
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  • Research Article   
  • J Phylogenetics Evol Biol 2017, Vol 5(2): 184
  • DOI: 10.4172/2329-9002.1000184

DNA Barcoding and Phylogenetic Relationships of Selected South Indian Freshwater Fishes Based on mtDNA COI Sequences

Manickam Raja* and Pachiappan Perumal
Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem - 636 011, Tamil Nadu, India
*Corresponding Author : Manickam Raja, Department of Biotechnology, School of Biosciences, Periyar University, Periyar Palkalai Nagar, Salem- 636 011, Tamil Nadu, India, Tel: +91 9894277036, Email: [email protected]

Received Date: Aug 22, 2017 / Accepted Date: Sep 12, 2017 / Published Date: Sep 15, 2017

Abstract

DNA barcoding is an effective tool for the identification of species representing diverse taxa especially through the sequence analysis of mitochondrial cytochrome c oxidase subunit I (COI) gene. In the present study, DNA barcodes were generated from 46 species of freshwater fishes covering the Orders Cypriniformes, Siluriformes, Synbranchiformes and Perciformes representing 30 genera under 9 families. All the samples were collected from diverse sites which also includes some endemic species. A total of 47 COI sequences were generated. After amplification and sequencing of 678 base pair fragment of COI, primers were trimmed which invariably generated a 635 base pair barcode sequence. The average Kimura two-parameter (K2P) distances within-species, genera, families, and orders were 0.32%, 8.40%, 14.50%, and 18.65%, respectively. DNA barcode discriminated congeneric species without any confusion. The present study strongly supports the efficiency of COI as an ideal marker for DNA barcoding of selected freshwater fishes.

Keywords: Barcoding; COI; Indian freshwater fishes; mtDNA; Phylogeny

Citation: Raja M, Perumal P (2017) DNA Barcoding and Phylogenetic Relationships of Selected South Indian Freshwater Fishes Based on mtDNA COI Sequences. J Phylogenetics Evol Biol 5: 184. Doi: 10.4172/2329-9002.1000184

Copyright: © 2017 Raja M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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