DNA DAMAGE ANALYSIS IN MAMMALIAN CELLS BY SMA-DMSO COMPLEX USING COMET ASSAY
|Pallavi Singh1,Chaiti Ganguly2, F.W.Bansode3
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SMA-DMSO complex (RISUG) is a potent anti fertility compound developed by Prof.S.K Guha at IITKharagpur. It has been fabricated as a male contraceptive which works by lowering the pH of vas deference in males and hence damaging the sperms.Cytotoxicity of SMA-DMSO was assessed using COMET assay whereby concentration of SMA-DMSO ranging from .001 Ã¯ÂÂM to 1 Ã¯ÂÂM was tested for inducing any significant DNA damage in Mammalian (Chinese Hamster Ovary) cell line. 50 cells were scored for each concentration, with 25 cells scored from each of two replicate blind coded slides. Statistical significance of the results was assessed by means of one-tailed Student’s t-test. Analysis of tail length and % DNA amount after treating the CHO cells with varying concentration of SMA-DMSO ,ranging from 0.001 Ã¯ÂÂm to 1 Ã¯ÂÂm has shown no significant DNA damage and cytotoxicity. Results have shown RISUG to be absolutely safe for futher usage in Mammalian cells.