DNA Damage and Cell Death Induced by Crude Extract of the Orchid Oncidium flexuosum SimsMônica Piacentini Luizon1, Caroline Santos Oliveira1, Danielly Kathe Zaqueo1, Jaqueline Aparecida Oliveira1, Gustavo Ligieri Pereira do Prado2, Renata Barbieri1, Fernanda Oliveira Gaspari de Gaspi1, João Ernesto de Carvalho3, Elaine Cristina Mathias da Silva-Zacarin4, Camila Andréa Oliveira5, Acácio Antônio Pigoso1 and Grasiela Dias de Campos Severi-Aguiar5*
2Departamento de Anatomia Patológica da Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campus Universitario Zeferino Vaz SN - Cidade Universitária - CEP 13083-970 Campinas, São Paulo, Brazil
5Programa de Pós-Graduação em Ciências Biomédicas, Centro Universitário Hermínio Ometto, UNIARARAS, Avenida Dr. Maximiliano Barutto, nº 500, Jd. Universitário, CEP 13607-339, Araras, São Paulo, Brazil
- *Corresponding Author:
- Severi Aguiar GDC
Centro Universitário Hermínio Ometto
UNIARARAS, Av. Maximiliano Barutto n° 500
13607-339, Araras, SP, Brazil
Tel: 55 (19) 3543 1474
Fax: 55 (19) 3543 1412
E-mail: [email protected]
Received date: June 12, 2013; Accepted date: July 26, 2013; Published date: July 28, 2013
Citation: Luizon MP, Oliveira CS, Zaqueo DK, Oliveira JA, do Prado GLP, et al. (2013) DNA Damage and Cell Death Induced by Crude Extract of the Orchid Oncidium flexuosum Sims. Med chem 3:247-253. doi:10.4172/2161-0444.1000147
Copyright: © 2013 Luizon MP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Oncidium flexuosum Sims. is an orchidwhose leaves are frequently used for therapeutic applications although its action mechanism is not exactly understood. So the aim of this study was investigate its effects in Wistar rat liver, kidney and bone marrow. For this purpose, twenty-five adult male Wistar rats were divided into five groups of 5 animals each: a control group receiving only water, a positive control receiving a mutagen compound and three groups treated for 15 days with 100, 250 or 500 mg/kg/day of a crude hydroalcoholic leaf extract of O. flexusosum Sims. No significant alterations were observed in –SH groups, MDA or catalase, showing that the extract does not induce hepatic oxidative damage. There was no evidence of kidney injury and hepatic function alteration (ALT). However, significant mitochondrial swelling was observed in the treated groups and is probably related to cell death induction observed in cells that displayed acidophilia, pyknosis and positive labeling with the TUNEL technique at higher concentrations of the extract. We demonstrated that the hepatotoxicity of the extract resulted from its ability to induce DNA and mitochondrial damage that arrests cells, leading to cell death, probably caused by the flavonoidsand tannins present in this extract.