alexa DNA Damage and Cell Death Induced by Crude Extract of the Orchid Oncidium flexuosum Sims | OMICS International | Abstract
ISSN: 2161-0444

Medicinal Chemistry
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

DNA Damage and Cell Death Induced by Crude Extract of the Orchid Oncidium flexuosum Sims

Mônica Piacentini Luizon1, Caroline Santos Oliveira1, Danielly Kathe Zaqueo1, Jaqueline Aparecida Oliveira1, Gustavo Ligieri Pereira do Prado2, Renata Barbieri1, Fernanda Oliveira Gaspari de Gaspi1, João Ernesto de Carvalho3, Elaine Cristina Mathias da Silva-Zacarin4, Camila Andréa Oliveira5, Acácio Antônio Pigoso1 and Grasiela Dias de Campos Severi-Aguiar5*

1NUCISA – Núcleo de Ciências da Saúde, Centro Universitário Hermínio Ometto, UNIARARAS, Avenida Dr. Maximiliano Barutto, nº 500, Jd. Universitário, Araras, São Paulo, Brazil

2Departamento de Anatomia Patológica da Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Campus Universitario Zeferino Vaz SN - Cidade Universitária - CEP 13083-970 Campinas, São Paulo, Brazil

3CPQBA, University of Campinas, P.O. Box 6171, 13083-970 Campinas, SP, Brazil

4Laboratório de Biologia Estrutural e Funcional (LABEF), Universidade Federal de São Carlos – UFSCAR, Rodovia João Leme dos Santos, Km 110 - SP-264, CEP 18052-780 Sorocaba, São Paulo, Brazil

5Programa de Pós-Graduação em Ciências Biomédicas, Centro Universitário Hermínio Ometto, UNIARARAS, Avenida Dr. Maximiliano Barutto, nº 500, Jd. Universitário, CEP 13607-339, Araras, São Paulo, Brazil

*Corresponding Author:
Severi Aguiar GDC
Centro Universitário Hermínio Ometto
UNIARARAS, Av. Maximiliano Barutto n° 500
Jardim Universitário
13607-339, Araras, SP, Brazil
Tel: 55 (19) 3543 1474
Fax: 55 (19) 3543 1412
E-mail: [email protected]

Received date: June 12, 2013; Accepted date: July 26, 2013; Published date: July 28, 2013

Citation: Luizon MP, Oliveira CS, Zaqueo DK, Oliveira JA, do Prado GLP, et al. (2013) DNA Damage and Cell Death Induced by Crude Extract of the Orchid Oncidium flexuosum Sims. Med chem 3:247-253. doi:10.4172/2161-0444.1000147

Copyright: © 2013 Luizon MP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Oncidium flexuosum Sims. is an orchidwhose leaves are frequently used for therapeutic applications although its action mechanism is not exactly understood. So the aim of this study was investigate its effects in Wistar rat liver, kidney and bone marrow. For this purpose, twenty-five adult male Wistar rats were divided into five groups of 5 animals each: a control group receiving only water, a positive control receiving a mutagen compound and three groups treated for 15 days with 100, 250 or 500 mg/kg/day of a crude hydroalcoholic leaf extract of O. flexusosum Sims. No significant alterations were observed in –SH groups, MDA or catalase, showing that the extract does not induce hepatic oxidative damage. There was no evidence of kidney injury and hepatic function alteration (ALT). However, significant mitochondrial swelling was observed in the treated groups and is probably related to cell death induction observed in cells that displayed acidophilia, pyknosis and positive labeling with the TUNEL technique at higher concentrations of the extract. We demonstrated that the hepatotoxicity of the extract resulted from its ability to induce DNA and mitochondrial damage that arrests cells, leading to cell death, probably caused by the flavonoidsand tannins present in this extract.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7