alexa DNA Detection Technology Using Zinc Finger Protein
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Review Article

DNA Detection Technology Using Zinc Finger Protein

Takenori Kumagai1, Koichi Abe1, Wataru Yoshida2 and Kuzunori Ikebukuro1*

1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan

2School of Biotechnology and Bioscience, Tokyo University of Technology,1404-1 Katakuramachi, Hachioji City, Tokyo, Japan

*Corresponding Author:
Kuzunori Ikebukuro
Department of Biotechnology and Life Science
Tokyo University of Agriculture and Technology
Tokyo, Japan
Tel: 81 42-367-5504
E-mail: [email protected]

Received Date: September 06, 2015; Accepted Date: September 14, 2015; Published Date: September 21, 2015

Citation: Kumagai T, Abe K, Yoshida W, Ikebukuro K (2015) DNA Detection Technology Using Zinc Finger Protein. J Microb Biochem Technol 7:278-281. doi:10.4172/1948-5948.1000225

Copyright: © 2015 Kumagai T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

With recent advances in nanotechnology and sequencing technology, DNA diagnostic technology is becoming practical and is advancing at a fast pace. In these detection systems, mainly PCR (particularly real-time PCR) and DNA probe hybridization techniques are used. We suggest that detecting PCR products using double-stranded DNA (dsDNA) is more convenient and powerful compared with DNA probe hybridization technique. Zinc finger protein is the major DNA binding protein in nature and it recognizes dsDNA with sequence specific manner. Additionally, by changing its amino acids sequence, we can design it to recognize the desired DNA sequence to some extent. Using zinc finger protein for DNA detection element, simple, accurate and sensitive DNA detection can be achieved. In this review, dsDNA detection using zinc finger protein is described and compared with recent advanced technology.

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