alexa DNA Methylation Signature of Post-injury Neointimal Cel
ISSN: 2168-9547

Molecular Biology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

DNA Methylation Signature of Post-injury Neointimal Cells During Vascular Remodeling in the Rat Balloon Injury Model

Jendai Richards1, Henry Ato Ogoe2, Wenzhi Li1, Oguljahan Babayewa1, Wei Xu1, Tameka Bythwood1, Minerva Garcia-Barrios1, Li Ma1,3* and Qing Song1,3*

1Cardiovascular Research Institute and Department of Medicine, Morehouse School of Medicine, Atlanta, Georgia, USA.

2Department of Biomedical Informatics, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

34DGenome Inc, Atlanta, Georgia, USA

*Corresponding Authors:
Qing Song
Cardiovascular Research Institute, Morehouse School of Medicine
720 Westview Drive SW, Atlanta, GA 30310, USA
Tel: 404-752-1845
E-mail: [email protected]

Li Ma
Cardiovascular Research Institute, Morehouse School of Medicine
720 Westview Drive SW, Atlanta, GA 30310, USA
Tel: 404-752-1845
E-mail: lma@ msm.edu

Received date: May 04, 2016; Accepted date: May 11, 2016; Published date: May 18, 2016

Citation: Richards J, Ogoe HA, Li W, Babayewa O, Xu W, et al. (2016) DNA Methylation Signature of Post-injury Neointimal Cells During Vascular Remodeling in the Rat Balloon Injury Model. Mol Biol 5:163. doi:10.4172/2168-9547.1000163

Copyright: © 2016 Richards J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Vascular smooth muscle cell (VSMC) accumulation in the neointimal is a common feature in vascular diseases such as atherosclerosis, transplant arteriosclerosis and restenosis. In this study, we isolated the neointimal cells and uninjured residential vascular smooth muscle cells by laser micro dissection and carried out single-cell whole-genome methylation sequencing. We also sequenced the bisulfite converted genome of circulating bone-marrow-derived cells such as peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC). We found totally 2,360 differential methylation sites (DMS) annotated to 1,127 gene regions. The majority of differentially methylated regions (DMRs) were located in intergenic regions, outside those CpG islands and island shores. Interestingly, exons have less DMRs than promotors and introns, and CpG islands contain more DMRs than islands shores. Pearson correlation analysis showed a clear clustering of neointimal cells with PBMC/BMMC. Gene set enrichment analysis of differentially methylated CpG sites revealed that many genes were important for regulation of VSMC differentiation and stem cell maintenance. In conclusion, our results showed that neointimal cells are more similar to the progenitor cells in methylation profile than the residential VSMCs at the 30th day after the vascular injury.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords