alexa Double-Staining Immunohistochemistry of Stem Cell Marke
ISSN: 2379-1764

Advanced Techniques in Biology & Medicine
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Research Article

Double-Staining Immunohistochemistry of Stem Cell Markers in Human Liver Cancer Tissues

Hiroyuki Tomita*, Kaori Tanaka, Kyoko Takahashi, Ayako Suga and Akira Hara
Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan
*Corresponding Author : Hiroyuki Tomita MD, PhD
Department of Tumor Pathology
Gifu University Graduate School of Medicine
1-1 Yanagido, Gifu, 501-1194, Japan
Tel: 81582306225
Fax: 81582306226
E-mail: [email protected]
Received April 06, 2016; Accepted April 14, 2016; Published April 21, 2016
Citation: Tomita H, Tanaka K, Takahashi K, Suga A, Hara A (2016) Double-Staining Immunohistochemistry of Stem Cell Markers in Human Liver Cancer Tissues. Adv Tech Biol Med 4:173. doi: 10.4172/2379-1764.1000173
Copyright: © 2016 Tomita H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Recently, Immunohistochemistry (IHC) using specific antibodies has been exploited to detect the localization of stem cells and cancer stem cells (CSCs). Typically, there is a very small population of stem cells in normal and cancerous tissues, making it difficult to investigate their cell type and localization in human specimens. Hence, for pathological detection of human specimens, IHC has certain advantages over immunofluorescence staining. In the present study, we established a method for double-staining cancer stem cell markers and other putative markers associated with human liver cancer. We have summarized and reviewed the methodology for IHC with doublestaining in human liver cancer tissues.

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