alexa Double-Staining Immunohistochemistry of Stem Cell Marke
ISSN: 2379-1764

Advanced Techniques in Biology & Medicine
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Double-Staining Immunohistochemistry of Stem Cell Markers in Human Liver Cancer Tissues

Hiroyuki Tomita*, Kaori Tanaka, Kyoko Takahashi, Ayako Suga and Akira Hara
Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan
*Corresponding Author : Hiroyuki Tomita MD, PhD
Department of Tumor Pathology
Gifu University Graduate School of Medicine
1-1 Yanagido, Gifu, 501-1194, Japan
Tel: 81582306225
Fax: 81582306226
E-mail: [email protected]
Received April 06, 2016; Accepted April 14, 2016; Published April 21, 2016
Citation: Tomita H, Tanaka K, Takahashi K, Suga A, Hara A (2016) Double-Staining Immunohistochemistry of Stem Cell Markers in Human Liver Cancer Tissues. Adv Tech Biol Med 4:173. doi: 10.4172/2379-1764.1000173
Copyright: © 2016 Tomita H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Recently, Immunohistochemistry (IHC) using specific antibodies has been exploited to detect the localization of stem cells and cancer stem cells (CSCs). Typically, there is a very small population of stem cells in normal and cancerous tissues, making it difficult to investigate their cell type and localization in human specimens. Hence, for pathological detection of human specimens, IHC has certain advantages over immunofluorescence staining. In the present study, we established a method for double-staining cancer stem cell markers and other putative markers associated with human liver cancer. We have summarized and reviewed the methodology for IHC with doublestaining in human liver cancer tissues.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version