DRB1*04 and DQB1*03 Alleles are Very Prevalent in Bahraini Families with Both T2DM and T1DM
|Einas M Al-Harbi1,2*#, Eman M Farid3#, Fayza A Junaid4, Jaipaul Singh5 and Khalid A Gumaa3|
|1Molecular Genetics Laboratory, Kuwait Medical Genetics Center, MOH, Kuwait|
|2Al-Jawhara Centre for Molecular Medicine, Arabian Gulf University, Manama, Bahrain|
|3College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain|
|4Department of Pediatrics, Salmaniya Medical Complex, MOH, Bahrain|
|5School of Forensic and Investigative Sciences and School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, UK|
|#Both authors have equally contributed in this work as first author|
|Corresponding Author :||Einas M Al-Harbi
Molecular Genetics Laboratory
Kuwait Medical Genetics Center Center
Ministry of Health-State of Kuwait, Kuwait
Tel: +965 55554666
Fax: +965 24842073
E-mail: [email protected]
|Received: December 17, 2014; Accepted: March 18, 2015; Published: March 25, 2015|
|Citation: Al-Harbi EM, Farid EM, Junaid EA, Singh J, Gumaa KA (2015) DRB1*04 and DQB1*03 Alleles are Very Prevalent in Bahraini Families with Both T2DM and T1DM. J Clin Cell Immunol 6:309. doi:10.4172/2155-9899.1000309|
|Copyright: © 2015 Al-Harbi EM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Objectives: Several investigations in families with both T1DM and T2DM have been reported to elucidate the genetic interaction between T1DM and T2DM and the clinical consequences for both diseases. The frequent occurrence of T1DM in relatives of patients with T2DM has also been previously observed. This study investigated whether the T2DM parent/grandparents share a specific HLA class II-DRB1and DQB1 alleles and their haplotype combination with the T1DM child.
Methods: Twenty four Bahraini families with a T1DM child and either parents or grandparents with T2DM and with no family history of T1DM were selected. HLA class II-DRB1 and DQB1 were examined by SSP-PCR method and the distribution was analyzed.
Results: In relation to DRB1*, the most common shared allele was DRB1*04:01:01 83% (n=20), while the most common shared DQB1* allele was DQB1*03:02:01 83% (n=20). In addition, the most common shared haplotype was DRB1*04:01:01-DQB1*03:02:01 83% (n=20).
Conclusions: The current study showed that DR4 and DQ3 alleles and its haplotype combination are the highest prevalence in the selected Bahraini families with mixed T1DM and T2DM patients. T2DM parents possessing this haplotype are more likely to have a child with T1DM, especially in families with no history of T1DM. The excess transmission of DR4-linked haplotypes from parents with T2DM to offspring with T1DM has been clearly observed in the present study.