alexa Effect of Alcohol Administration on Mg2+ Homeostasis in H9C2 Cells
ISSN: 2329-9517

Journal of Cardiovascular Diseases & Diagnosis
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Research Article

Effect of Alcohol Administration on Mg2+ Homeostasis in H9C2 Cells

Huy Nguyen and Andrea MP Romani*
Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Corresponding Author : Andrea MP Romani
Department of Physiology and Biophysics
Case Western Reserve University
10900 Euclid Avenue, Cleveland, OH 44106, USA
Tel: 216-3681625
Fax: 216-3685586
E-mail: [email protected]
Received August 19, 2014; Accepted October 16, 2014; Published October 23, 2014
Citation: Nguyen H, Romani AMP (2014) Effect of Alcohol Administration on Mg2+ Homeostasis in H9C2 Cells. J Cardiovasc Dis Diagn 2:179. doi: 10.4172/2329-9517.1000179
Copyright: © 2014 Nguyen H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Alcoholic cardiomyopathy represents one of the main clinical complications in chronic alcoholics. This pathology contrasts the seemingly beneficial effect of small doses of alcohol on the cardiovascular system. Studies carried out in liver cells exposed acutely or chronically to varying doses of EtOH indicate that intrahepatic alcohol metabolism results in a major loss of cellular Mg2+. To investigate whether EtOH administration also induced Mg2+ extrusion in cardiac cells, H9C2 cells were exposed to varying doses of EtOH for short- or ling-term periods of time. The results indicate that H9C2 cells exposed to EtOH doses higher than 0.1% (v/v, or 15 mM) extruded Mg2+ into the extracellular medium on a time- and dose-dependent manner. Consistent with the involvement of cyP4502E1 in metabolizing EtOH, administration of chloro-methiazole (CMZ) as an inhibitor of the cytochrome prevented EtOHinduced Mg2+ loss to a large extent. EtOH-induced Mg2+ extrusion was also prevented by the administration of di-thiotreitol (DTT) and n-acetyl-cysteine (NAC), two agents that prevent the negative effects of ROS formation and free radicals generation associated with EtOH metabolism by cyP4502E1. Taken together, our data indicate that Mg2+ extrusion also occur in cardiac cells exposed to EtOH as a result of alcohol metabolism by cyP4502E1 and associated free radical formation. Interestingly, Mg2+ extrusion only occurs at doses of EtOH higher than 0.1% administered for an extended period of time. The significance of Mg2+ extrusion for the onset of alcoholic cardiomyopathy remains to be elucidated.


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