Effect of Application Times on Candida albicans for Denture Disinfection
|Department of Prosthetic Dentistry, University Medical Center Regensburg, Germany|
|Corresponding Author :||Ralf Buergers, DDS, PhD
Department of Prosthetic Dentistry
University Medical Center Regensburg
93042 Regensburg, Germany
E-mail: [email protected]
|Received October 29, 2011; Accepted December 04, 2011; Published December 06, 2011|
|Citation: Buergers R (2011) Effect of Application Times on Candida albicans for Denture Disinfection. Fungal Genom Biol 1:101. doi:10.4172/2165-8056.1000101|
|Copyright: © 2011 Buergers R. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Study background: This in vitro study aimed to evaluate the minimum application times of three denture
disinfection procedures to significantly reduce initial Candida albicans colonization.
Methods: Specimens of a soft denture relining material were incubated with Candida albicans for 2.5 h
and allotted to three different disinfection regimes (sodium hypochlorite 1%, effervescent cleansing tabs, and microwaving immersed in water) with application times ranging from 30 to 600 s. Adhering fungi were quantified using a bioluminescence assay in combination with an automated plate reader for cell quantification.
Results: Luminescence at baseline varied between 409 and 472 relative luminescence units (rlu). A significant reduction of luminescence was found after 120 s of soaking in sodium hypochlorite (31 rlu) and effervescent cleansing tabs (35 rlu), and after 60 s of microwave irradiation (26 rlu).
Conclusions: Threshold minimum application times of 120 s for sodium hypochlorite and cleansing tabs, and 60 s for microwaving were determined. A further extension of application times did not have additional antifungal effects.