Effect of Argan Oil-Hydrogen Peroxide Mixture on Mycobacterium tuberculosis- In Vitro
Anwar K. AL-Saffar* and Hussein O. M. Al-Dahmoshi
Babylon University-College of Science, Biology Department, Hilla-Iraq
- *Corresponding Author:
- Anwar Kadhim Hussein AL-Saffar
Babylon University-College of Science
Biology Department, Babylon-Hilla City, Iraq
E-mail: [email protected]
Received date: May 01, 2015; Accepted date: June 28, 2015; Published date: June 30, 2015
Citation: AL-Saffar AK, Al-Dahmoshi HOM (2015) Effect of Argan Oil-Hydrogen Peroxide Mixture on Mycobacterium tuberculosis-In Vitro. J Med Microb Diagn S3:008. doi:10.4172/2161-0703.S3-008
Copyright: © 2015 AL-Saffar AK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: Tuberculosis (TB) is the threatful infectious disease that dwell about one third of worlds. Among Middle East, Iraq occupy the fifth order country endemic with TB.
Methodology: Six mixtures of Argan oil: 1.5% H2O2 were prepared at different ratio: 1=0.75:9.25, 2=1:9, 3=1.5:8.5, 4=2:8, 5=2.5:7.5, 6=3:7 and mixed with Lowenstein-Jensen (L-J) medium. Seventeen Mycobacterium tuberculosis isolates got from the consultants clinic of respiratory and thoracic diseases in Hilla city-Iraq. All isolates cultured on LJ medium containing the mixture of Argan oil: 1.5% H2O2 in different ratio and control tube (LJ without mixture) also used. The growth monitored per a day and recorded at different interval and the inhibition (no growth) were recorded.
Results: The effect of Argan oil: 1.5% H2O2 mixture on the Mycobacterium tuberculosis were investigated over three incubation intervals (14th -20th, 21st -28th and after 29th day). The results revealed the high Argan concentration mixture 2.5:7.5 and 3:7 give excellent inhibitory effect on Mycobacterium tuberculosis during full periods of incubation with inhibition percentage (64.71%-82.35%).
Conclusion: The current study aims to investigate the effects of argan oil on Mycobacterium tuberculosis and conclude the anti-mycobacterial activity of argan oil as a safe medication for prophylaxis and treatment of TB.