Effect of different fluoride concentrations on remineralization of demineralized enamel: an in vitro pH-cycling study
Objectives. The purpose of this study was to determine the effects of three different fluoride mouth rinses (226, 450 and 900 ppm) in comparison to non-fluoride application group (control group) on demineralized enamel under in vitro pH-cycling conditions.
Methods. Initial demineralization was obtained by acetic acid for 24 hours. After remineralization for 11.5 h, pH-cyclus was as follows: demineralization with acid solution for 30 min., application of NaF (control (0), 226, 450 and 900 ppm F-) for 2 min. and remineralization for 11.5 h. This procedure was applied twice. This 24-hour cycling application was repeated for 28 days. Vickers microhardness measurements were conducted at the beginning, after the initial demineralization and after 3, 7, 14 and 28 days pH-cycling applications.
Results. Remineralization begins after 14 days in all groups (Wilcoxon, p > 0.05). Only the group with 226 ppm fluoride reached the beginning microhardness (p > 0.05).
Conclusions. It was concluded that regular daily use of fluoride solutions with 226 ppm F- enhanced
remineralization in the pH-cyclus environment and reached the beginning microhardness.
Demineralization did not continue in any fluoride treatment group, even in the control group.