Immunotherapy: Open Access
Open Access

Abstract

Effect of Genetic Polymorphisms in CD14 and TLR4 on Cardiomyopathy

Avinash Bardiaa, Siddhratha Rout, Sandeep Kumar Vishwakarma, Chandrakala Lakki Reddy, Shaik Iqbal Ahmed, Pratibha Nallari and Aleem A Khan

Background: We investigated polymorphisms of immune related genes (CD14 and TLR4) and explore the associations of these variants and risk of CM.

Method: We detected SNPs of CD14 (-159C>T) and TLR4 (299 A>G) using the polymerase chain reaction (PCR) with peripheral blood samples from 141 patients with CM and 198 healthy people, further analyzing their relations with the risk of CM.

Result: The CT genotype of CD14 gene was found to be associated with CM and 1.84 folds increased risk to CM when compared to CC genotype (OR 1.84, 95% CI 1.18–2.88, P=0.0051). Heterozygotes (AG) of TLR4 gene were found to be predominant in the CM group when compared to controls (50.4%, 38.4% respectively, P=0.0081) with 1.79 folds increased risk for CM, which was statistically significant (OR 3.79, 95% CI 1.15–2.80, P=0.0081).

Conclusion: Polymorphism in CD14 and TLR4 gene is associated with an increased risk of CM. The combined effects of polymorphisms within innate immune genes of CD14 and TLR14 may contribute to a high risk of CM.