Effect of Haemostatic Agent Application on the Shear Bond Strength of Contemporary/Multi-Mode Adhesive Systems
Objectives: The aim was to determine the effect of blood contamination and haemostatic agent application on the shear bond strength (SBS) of different adhesives to dentin.
Materials and Methods: Seventy-two extracted human molars were used in this study. Teeth were mounted acrylic in moulds. Mesial and distal surfaces were removed to obtain flat dentin surfaces (n=144) and grinded with a 600 grit sandpaper. The samples were randomly divided into three main groups (N=48) based on the adhesive system and application procedures. Group 1: No contamination (control), Group 2: Blood contamination, Group 3: Blood Contamination+Haemostatic agent application. Each group was further divided into four subgroups: Subgroup I: Single Bond 2 (etch-and-rinse) Subgroup II: Clearfil SE Bond (two-step self-etch) Subgroup III: Single Bond Universal (multimode, etch-and-rinse) Subgroup IV: Single Bond Universal (multimode, all-in-one self-etch) (n=12). Resin cylinders (Filtek Z550) were bonded to the dentin surfaces according to the manufacturers’ instructions. A shear load was applied to the specimens using universal testing machine at a cross-head speed of 0.5 mm/min until failure. Data were analyzed statistically (p<0.05).
Results: Statistically significant differences were observed between no contamination (control) and blood + haemostatic agent contamination groups for both Single Bond 2 (etch-and-rinse) and Single Bond Universal (multimode, all-in-one self-etch) (p<0.05). When the adhesive systems were compared to each other, no statistically significant differences were found in all contamination groups and control group for the mean SBS values (p>0.05).
Conclusion: When blood contamination is inevitable two step self- etching adhesive systems may be the choice of adhesive system in terms of shear bond strength.