Effect of L-arginine on Function of Mitochondria in Ischemia Ã¢ÂÂ Reperfusion Myocardial Cell in RabbitsChen D1, Huang L1, Jinbo He1, Song D1, Yingchun Ma1, Lina Lin2* and Wang W1*
- *Corresponding Author:
- Wantie Wang
Department of Pathophysiology, Wenzhou Medical University
Wenzhou 325025, Zhejiang, P.R. China
Fax: 0577-86689817; E-mail: [email protected]
Department of Anesthesiology
the First Affiliated Hospital of Wenzhou Medical University
Wenzhou 325003, Zhejiang, P.R.China
Tel: +86-577- 88069459
E-mail: [email protected]
Received date: February 17, 2014; Accepted date: June 25, 2014; Published date: June 27, 2014
Citation: Chen D, Huang L, Jinbo He, Song D, Yingchun Ma, et al. (2014) Effect of L-arginine on Function of Mitochondria in Ischemia – Reperfusion Myocardial Cell in Rabbits. J Cell Sci Ther 5: 166. doi: 10.4172/2157-7013.1000166
Copyright: © 2014 Chen D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: To investigate the effect of L-arginine (L-Arg) on the function of myocardial mitochondria during myocardia ischemia - reperfusion (IR). Methods: Dividing randomly the 30 rabbits into three groups (n=10): control group (C), myocardia ischemiareperfusion group (IR) and L-Arginine pretreatment group (L-Arg+IR). The relevant parameters, including myocardial mitochondria respiratory function, Ca2+ concentration ([Ca2+]), malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity, myocardial adenosine triphosphate (ATP), Adenosine diphosphate (ADP), adenosine monophosphate (AMP) content, the total amount of AMP (TAN, TAN=ATP+ADP+AMP) and energy charge (EC, EC=1/2ADP+ATP/TAN) were determined, respectively. Results: The mitochondria respiratory control rate (RCR), III state respiration rate (V3), and SOD in group L-Arg +IR were significantly higher than those in group IR, while IV state respiration rate (V4) , [Ca2+] m, and MDA were obviously lower than those of group IR, the levels of ATP, ADP, TNA and the EC of myocardium showed significantly higher than those in group IR. There were no significant differences in terms of V3, V4, SOD, MDA, AMP and TAN between group L-Arg+IR and group C. Conclusion: It indicated that L-arginine can reduce the level of the oxygen free radicals and partly attenuatecalcium overload to improve the function of myocardial mitochondria during myocardium ischemia reperfusion injury.