alexa Effect of Lignin Glycosides Extracted from Pine Cones on the Human SIRT1 Promoter
ISSN : 2153-2435

Pharmaceutica Analytica Acta
Open Access

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Research Article

Effect of Lignin Glycosides Extracted from Pine Cones on the Human SIRT1 Promoter

Fumiaki Uchiumi1,2,Haruki Tachibana3,Steven Larse2 and Sei-ichi Tanuma2,3,4,5*

1Department of Gene Regulation,Faculty of Pharmaceutical Sciences,Tokyo University of Science,Noda-shi, Chiba-ken,Japan

2Research Center for RNA Science,RIST,Tokyo University of Science,Noda-shi,Chiba-ken,Japan

3Department of Biochemistry,Faculty of Pharmaceutical Sciences,Tokyo University of Science,Noda-shi,Chiba-ken,Japan

4Genome and Drug Research Center, Tokyo University of Science, Noda-shi, Chiba 278-0022, Japan

5Drug Creation Frontier Research Center, RIST, Tokyo University of Science, Noda-shi, Chiba 278-0022, Japan

*Corresponding Author:
Sei-ichi Tanuma, Ph.D
Department of Biochemistry
Faculty of Pharmaceutical Sciences
Tokyo University of Science, 2641 Yamazaki
Noda-shi, Chiba-ken 278-8510, Japan
Tel: +81-4-7124-1501
Fax: +81-4-7121-3620
E-mail: [email protected]

Received Date: December 27, 2011; Accepted Date: January 28, 2012; Published Date: January 30, 2012

Citation: Uchiumi F, Tachibana H, Larsen S, Tanuma SI (2013) Effect of Lignin Glycosides Extracted from Pine Cones on the Human SIRT1 Promoter. Pharm Anal Acta 4: 266 doi: 10.4172/2153-2435.1000266

Copyright: © 2012 Uchiumi F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Sirtuins (SIRT1-7) and telomerase (TERT) are known to be involved in the regulation of cellular senescence/ aging processes. Resveratrol (Rsv), a natural polyphenol, which has been shown to extend the lifespan of diverse range of species. Furthermore, Rsv has been shown to activate SIRT1, the class III NAD+ dependent histone deacetylases (HDACs), directly in mammalian cells. Here, we examined the changes in promoter activities of the human SIRT1 and TERT genes after treatment with Rsv and lignin glycosidases/lignin carbohydrate complexes (LCC) extracted from pine cones of Pinus Karalensis. We constructed Luciferase (Luc) expression vectors, pGL4- SIRT1 and pGL4-TERT, containing 396-bp and 263-bp of the 5’-upstream regions of human SIRT1 and TERT genes, respectively, which were then transfected into HeLa S3 cells. The Luc reporter plasmid assay revealed that treatment with the LCC increases not only SIRT1 but also TERT promoter activities more than that with Rsv. These results suggest that the LCC from pine cones induces expression of both SIRT1 and TERT genes, which is thought to have anti-aging effects.

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