Effect of Methotrexate on the Jejunum of Adult Albino Rat and the Possible Protective Role of Vitamin A: Histological and Immunohistochemical Study
Mohamed BM Ali, Maha H Ashmawy, Ehab A A El-shaarawy*, Inas A Mohamed and Tarek I Abd El-Galil
Department of Anatomy, Faculty of Medicine, Cairo University, Egypt
- *Corresponding Author:
- Ehab Abdel Aziz Ahmed El-Shaarawy
Department of Anatomy, Faculty of Medicine
Cairo University, Egypt
E-mail: [email protected]; [email protected]
Received Date: June 03, 2014; Accepted Date: July 12, 2014; Published Date: July 14, 2014
Citation: Ali MBM, Ashmawy MH, El-shaarawy EAA, Mohamed IA, El-Galil TIA (2014) Effect of Methotrexate on the Jejunum of Adult Albino Rat and the Possible Protective Role of Vitamin A: Histological and Immunohistochemical Study. J Cytol Histol 5:263. doi:10.4172/2157-7099.1000263
Copyright: © 2014 Ali MBM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Methotrexate (MTX) is an antineoplastic drug that is widely used in the treatment of neoplasms. Patients undergoing MTX therapy have a variety of side effects affecting many important organs.
Aim of the work: The aim of the present study was to detect the histological and the immunohistochemical adverse alterations that might occur in the jejunum of the adult albino rats following methotrexate therapy and to determine the possible protective role of vitamin A. Material and
Methods: Seventy two adult male albino rats were divided into six equal groups. In all groups the animals were sacrificed 24 hours after the last injection. The jejunal specimens were examined using H& E and Masson’s trichrome stains and immunohistochemical examination to study the alkaline phosphatase activity using light microscopy. Morphometric study measuring villous height, crypt depth, number of goblet cells, percentage area of fibrosis and the optical density of alkaline phosphatase activity was done in all groups.
Results: The methotrexate treated groups’ revealed different changes in the jejunum of rats. Distortion and cystic dilatation of the crypts, cellular shedding and dilated blood vessels were found. Morphometric study showed decreased villus height and goblet cells, increased the crypts depth. Immunohistochemical examination of the jejunal sections of the methotrexate treated groups showed weak reaction of alkaline phosphatase enzyme when compared with that of the control group.
Conclusion: It can be concluded that the toxic effect of methotrexate on the jejunum of rats can be partially improved with the concomitant use of vitamin A.