alexa Effect of Photodynamic Therapy with Different Formulations of Methylene Blue in Teeth Contaminated by Enterococcus faecalis
ISSN: 2161-0398

Journal of Physical Chemistry & Biophysics
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Research Article

Effect of Photodynamic Therapy with Different Formulations of Methylene Blue in Teeth Contaminated by Enterococcus faecalis

Eliana Barbosa de Souza1*, Maria Regina Lorenzetti Simionato2, José Luiz Lage-Marques1 and João Humberto Antoniazzi1
1Restorative Dentistry Department, Faculty of Dentistry, University of Sao Paulo, Brazil
2Department of Oral Microbiology, Biomedical Sciences Institute – University of Sao Paulo, Brazil
Corresponding Author : Eliana Barbosa de Souza
Restorative Dentistry Department, Faculty of Dentistry
University of Sao Paulo, Brazil
Tel: 55 11 30917839
E-mail: [email protected]
Received August 01, 2013; Accepted August 26, 2013; Published August 28, 2013
Citation: de Souza EB, Lorenzetti Simionato MR, Lage-Marques JL, Antoniazzi JH (2013) Effect of Photodynamic Therapy with Different Formulations of Methylene Blue in Teeth Contaminated by Enterococcus faecalis. J Phys Chem Biophys 3:124. doi:10.4172/2161-0398.1000124
Copyright: © 2013 de Souza EB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The aim of this study was to compare the disinfection of dentine using photodynamic therapy with methylene blue in different formulations. Thirty bovine teeth roots were autoclaved and incubated with a suspension of Enterococcus faecalis. The specimen were randomly divided into three groups: G1, the roots were filled with 10 mM methylene blue dissolved in water; G2, the roots were filled with 10 mM methylene blue dissolved in a mixture of glycerol: ethanol: water; G3, roots filled with 100 mM methylene blue dissolved in water. The groups were irradiated with a 660 nm diode laser with an output power of 100 mW for 4 min, energy density of 850 J/cm2 and after this procedure, the sensitizer was removed and microbial samples were collected from within the root canals. The samples were plated on mEnterococcus to count the colony-forming units (CFU/mL). The means were: Group 1=513×103, Group 2=1431×103 and Group 3=2.96×103. The statistical analysis detected higher disinfection achieved by G3 when compared with groups G1 and G2, and no significant difference between the groups G1 and G2 (P>0.05). The increase of the concentration of methylene blue dye achieved higher disinfection in photodynamic therapy.

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