Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In VitroChikako Yoshikawa1, Fumihide Takano2,3, Yasuhito Ishigaki4, Masahiko Okada1, Satoru KYO1, Nobutaka Suzuki5, Kouichi Sugiura6 and Koji Koike5,7*
- *Corresponding Author:
- Koji Koike
Department of Complementary and Alternative Medicine
Kanazawa University Graduate School of Medical Science
Kanazawa, Ishikawa 920-8640, Japan
E-mail: [email protected]
Received date: October 23, 2013; Accepted date: November 24, 2013; Published date: November 30, 2013
Citation: Yoshikawa C, Takano F, Ishigaki Y, Okada M, KYO S, et al. (2013) Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro. Gynecol Obstet 3:186 doi: 10.4172/2161-0932.1000186
Copyright: © 2013 Yoshikawa C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Placental Extract (PE) has been used as a folk remedy in Asian countries. Human PE is clinically used for the treatment of skin wrinkles because it promotes skin regeneration. In this study, we found that Porcine PE (PPE) promoted collagen production in Normal Human Dermal Fibroblasts (NHDFs). Treatment of NHDFs with PPE at concentrations of 12.5-100 μg/mL enhanced their proliferation and secretion of procollagen C peptide. We also found that expression of intracellular Matrix Metalloproteinase-9 (MMP-9) in PPE-treated NHDFs was decreased in a dose-dependent manner. These results indicate that PPE might affect facial wrinkling partly by enhancing fibroblast proliferation and collagen secretion while inhibiting MMP-9 expression in NHDFs.