Effect of Protein Supplementation on in vitro Maturation of Sheep Oocytes and in vitro Culture of Preimplantation with α-Tocopherol Supplementation in CR1aa Medium on Sheep Embryos to the Blastocyst StageDulam Sreenivas1, Kaladhar DSVGK2*, Nagendra Sastry Yarla2, Thomas VM1 and PalniSamy A3
- *Corresponding Author:
- Kaladhar DSVGK
GITAM Institute of Science
GITAM University, Visakhapatnam
Andhra Pradesh-530045, India
E-mail: [email protected]
Received date: March 21, 2013; Accepted date: April 26, 2013; Published date: May 02, 2013
Citation: Sreenivas D, Kaladhar DSVGK, Yarla NS, Thomas VM, PalniSamy A (2013) Effect of Protein Supplementation on in vitro Maturation of Sheep Oocytes and in vitro Culture of Preimplantation with a-Tocopherol Supplementation in CR1aa Medium on Sheep Embryos to the Blastocyst Stage. J Aller Ther 4:133. doi: 10.4172/2155-6121.1000133
Copyright: © 2013 Sreenivas D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sheep play a vital role within the Indian economy and therefore the production from native breeds is comparatively low because of their poor generative potency. A great challenge of analysis is concerned to beat the constraints within the technology like costly and sophisticated nature of the technology and low success rate. Fetal bovine serum can be supplemented in IVM and IVF media to achieve better maturation and cleavage. Wheat peptone may not produce desired maturation and cleavage when compared to FBS and BSA, and may not be a useful supplement in IVM and IVF. BSA supplementation also yields better maturation and embryo development when compared to wheat peptone but would involve high cost in embryo production. Supplementation of wheat peptones in IVC of embryos yielded better embryo production suggesting that wheat peptones can be an alternative to animal protein in IVC and would help to minimize the cost in embryo production. The studies have shown that CR1aa medium with α-tocopherol at 200 μM is a better media for in vitro embryo production in sheep.