Effect of Staphylococcus aureus Infection on Biochemical and Antioxidant Activities of Fifth Instar Silkworm Larvae (Bombyx mori L.)
Harinatha Reddy A* and Venkatappa B
Department of Microbiology, Sri Krishnadevaraya University, Anantapur-515003, Andhra Pradesh, India
- *Corresponding Author:
- Harinatha Reddy A
Department of Microbiology, Sri Krishnadevaraya University
Anantapur-515003, A.P, India
E-mail: [email protected]
Received date: August 09, 2016; Accepted date: August 29, 2016; Published date: August 31, 2016
Citation: Reddy HA, Venkatappa B (2016) Effect of Staphylococcus aureus Infection on Biochemical and Antioxidant Activities of Fifth Instar Silkworm Larvae (Bombyx mori L.). J Bacteriol Parasitol 7:286. doi:10.4172/2155-9597.1000286
Copyright: © 2016 Reddy AH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In the present study silkworm used as a model animal for evaluating the pathogenicity of Staphylococcus aureus. The fifth instar silkworm larvae was used and infected by intrahaemocoelic injection of bacterial sample. The haemolymph was collected from the infected and control group larvae at 6, 12, 18 and 24 hours of post infection and stored at -4°C in eppendorf tubes to use. Lipid peroxidation, phenol oxidase and acid phosphatase activity were estimated in the haemolymph of control and infected group. This indicated that there was a gradual increase in lipid peroxidation, phenol oxidase and acid phosphatase activities in infected group when compared with control group. The antioxidant enzyme activities were estimated in the haemolymph of control and infected group. We found that the antioxidant enzyme activities were decreased after 24 hours of infection with S. aureus. The silk glands were removed and the wet weight was measured, the wet weight of the silk glands was decreased on the 24 hours of infection when compared with control group.