Effect of Stressed and Unstressed Cell Culture Environments on the Viability of MC3T3 Cells with Calcium Phosphates
- Corresponding Author:
- Knabe-Ducheyne C
Department of Experimental Orofacial Medicine
Faculty of Dentistry
Philipps University, Marburg Germany
E-mail: [email protected]
Received date: June 08, 2013; Accepted date: July 27, 2013; Published date: August 22, 2013
Citation: Lopez-Heredia MA, Gildenhaar R, Berger G, Linow U, Gomes C, et al. (2013) Effect of Stressed and Unstressed Cell Culture Environments on the Viability of MC3T3 Cells with Calcium Phosphates. Bioceram Dev Appl S1:002. doi:10.4172/2090-5025.S1-002
Copyright: © 2013 Lopez-Heredia MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The effect of Fetal Bovine Serum (FBS) deprivation on survival and apoptosis of osteoblasts cultured on various calcium phosphates was studied. Test materials were two calcium alkali orthophosphates (materials denominated: GB9 and GB14), which were compared to β-tricalcium phosphate (TCP). Tissue culture polystyrene (PS) served as control. Test materials were characterized by X-Ray Diffraction and scanning electron microscopy. An apoptotic challenge assay entailing serum withdrawal was applied: MC3T3-E1 osteoblasts were cultured for 72h on the test materials in serum containing medium, followed by incubation in serum free medium for another 24h. Serum withdrawal is an apoptotic challenge, which creates a stressed environment. Cells cultured on the test specimens in serum containing medium served as control. The TUNEL Assay was employed to quantify the percentage of apoptotic cells. GB9 and GB14 displayed a significantly lower percentage of apoptotic cells than TCP. TCP had significantly fewer apoptotic cells than PS. The percentage of apoptotic cells on GB9 and GB14 was less than 10%, while the number of apoptotic cells found on the untreated control specimens ranged between 5 and 7%. These findings indicate that GB9 and GB14 endow osteoblasts cultured on them with a decreased sensitivity to apoptosis, which corresponds well with the results of previous in vitro and in vivo studies.