Effect of Uterine Flushing Leukemia Inhibitory Factor on Reproductive Outcome of Infertile CoupleEbtesam M Kamal* and Abber M Hafez
Faculty of Medicine, Zagazig University, Egypt
- *Corresponding Author:
- Ebtesam M kamal
Faculty of Medicine
Zagazig University, Egypt
E-mail: [email protected]
Received Date: January 09, 2014; Accepted Date: January 29, 2014; Published Date: January 31, 2014
Citation: Kamal EM, Hafez AM (2014) Effect of Uterine Flushing Leukemia Inhibitory Factor on Reproductive Outcome of Infertile Couple. Gynecol Obstet (Sunnyvale) 4:201. doi: 10.4172/2161-0932.1000201
Copyright: © 2014 Kamal EM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Aim: To determine the role of Leukemia Inhibitory Factor (LIF) in predicting the reproductive outcome in infertile couple.
Method of study: Uterine flushing and endometrialsamples were collected from infertile women during implantation window with stage I/II endometriosis (n=17), patients with idiopathic infertility (n=24), luteal phase defect (n=16), and fertile control (n=26). LIF was assessed in uterine flushings in all patients by ELISA. In endometrium, semiquantitative RT-PCR was performed for LIF mRNA expression. Reproductive outcome of all infertile women were recorderd.
Results: 34.6% of patients included in the study got pregnant. LIF concentration had 96.5% sensitivity and 86.1% specificity at a cut-off point of 2.45 pg/ml for predicting the reproductive outcome.
Conclusion: LIF in uterine flushing could be used as a predictor of successful reproductive outcome in infertile women.