Effect of Vanadium on Splenocyte Apoptosis in BroilersCui Wei, Cui Hengmin*, Peng Xi, Fang Jing, Liu Xiaodong and Wu Bangyuan
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China
- *Corresponding Author:
- Cui Hengmin Ph.D
Professor, College of VeterinaryMedicine
Sichuan Agricultural University, Yaan
Sichuan, China 625014
E-mail: [email protected],[email protected]
Received date: March 14, 2012; Accepted date: March 30, 2012; Published date: April 02, 2012
Citation: Wei C, Hengmin C, Xi P, Jing F, Xiaodong L, et al. (2012) Effect of Vanadium on Splenocyte Apoptosis in Broilers. Med chem 2:057-060. doi:10.4172/2161-0444.1000115
Copyright: © 2012 Wei C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A total of 420 one-day-old healthy broilers were divided into six groups. There were 70 broilers in each group. The broilers were fed on a corn-soybean basal diet as a control diet (vanadium 0.073 mg/kg) or the same diet amended to contain 5 mg/kg, 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg vanadium supplied as ammonium metavanadate for 42 days. When compared with those of the control group, the mitochondrial injury of splenocytes and more apoptotic splenocytes with condensed chromatin with C-shaped, horseshoe-like, petal-shaped or crescent were ultrastructurally observed in the spleen in the 30 mg/kg, 45 mg/kg and 60 mg/kg groups. As measured by flow cytometry (FCM), the number of apoptotic splenocytes was significantly increased in the 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg groups. Immunohistochemical tests showed that number of positive splenocytes containing Bax and caspase-3 protein was increased, and number of positive splenocytes containing Bcl-2 protein was decreased in the 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg groups. It was concluded that dietary vanadium in excess of 15 mg/kg could result in splenocyte apoptosis in broilers. Splenocyte apoptosis was closely related to mitochondrial injury and changed expression of apoptogenic proteins induced by vanadium.