alexa Effect of Vanadium on Splenocyte Apoptosis in Broilers
ISSN: 2161-0444

Medicinal Chemistry
Open Access

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Research Article

Effect of Vanadium on Splenocyte Apoptosis in Broilers

Cui Wei, Cui Hengmin*, Peng Xi, Fang Jing, Liu Xiaodong and Wu Bangyuan

Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China

*Corresponding Author:
Cui Hengmin Ph.D
Professor, College of VeterinaryMedicine
Sichuan Agricultural University, Yaan
Sichuan, China 625014
Tel:+86-13608264628
Fax: +86-0835-2882340
E-mail: [email protected],[email protected]

Received date: March 14, 2012; Accepted date: March 30, 2012; Published date: April 02, 2012

Citation: Wei C, Hengmin C, Xi P, Jing F, Xiaodong L, et al. (2012) Effect of Vanadium on Splenocyte Apoptosis in Broilers. Med chem 2:057-060. doi:10.4172/2161-0444.1000115

Copyright: © 2012 Wei C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A total of 420 one-day-old healthy broilers were divided into six groups. There were 70 broilers in each group. The broilers were fed on a corn-soybean basal diet as a control diet (vanadium 0.073 mg/kg) or the same diet amended to contain 5 mg/kg, 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg vanadium supplied as ammonium metavanadate for 42 days. When compared with those of the control group, the mitochondrial injury of splenocytes and more apoptotic splenocytes with condensed chromatin with C-shaped, horseshoe-like, petal-shaped or crescent were ultrastructurally observed in the spleen in the 30 mg/kg, 45 mg/kg and 60 mg/kg groups. As measured by flow cytometry (FCM), the number of apoptotic splenocytes was significantly increased in the 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg groups. Immunohistochemical tests showed that number of positive splenocytes containing Bax and caspase-3 protein was increased, and number of positive splenocytes containing Bcl-2 protein was decreased in the 15 mg/kg, 30 mg/kg, 45 mg/kg and 60 mg/kg groups. It was concluded that dietary vanadium in excess of 15 mg/kg could result in splenocyte apoptosis in broilers. Splenocyte apoptosis was closely related to mitochondrial injury and changed expression of apoptogenic proteins induced by vanadium.

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