alexa Effect of Vitrified Frozen-Thawed Embryo Transplantatio
ISSN: 2153-0769

Metabolomics:Open Access
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Research Article

Effect of Vitrified Frozen-Thawed Embryo Transplantation on Glycometabolism of the Offspring Mice

Sui L1, Xu J1, Fu H1, Wu R1, Zhou J1, Zhou J1, Lu J2, Chen L1* and Yao B1*

1Center of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China

2Department of Laboratory Science, Nanjing Hospital, Jiangsu Crops, The Armed Police Force, PLA, Nanjing, China

*Corresponding Author:
Yao B
Center of Reproductive Medicine, Jinling Hospital
Nanjing University School of Medicine
Nanjing 210002, China
Tel: +86 2580863174
Fax: +86 2580863174
E-mail: [email protected]

 
Chen L
Center of Reproductive Medicine
Jinling Hospital, Nanjing University School of Medicine
Nanjing 210002, China
Tel: +86 2580863174
Fax: +86 2580863174
E-mail: [email protected]

Received Date: January 30, 2016 Accepted Date: February 15, 2016 Published Date: February 17, 2016

Citation: Sui L, Xu J, Fu H, Wu R, Zhou J, et al. (2016) Effect of Vitrified Frozen-Thawed Embryo Transplantation on Glycometabolism of the Offspring Mice. Metabolomics 6:167. doi: 10.4172/2153-0769.1000167

Copyright: © 2016 Sui L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Objective: To investigate the change of glycometabolism in the offspring from in vitro fertilization through fresh embryo and vitrified embryo transplantation, and evaluate the effect of vitrification technology on the health of offspring. Materials and method: Offspring mice from fresh embryo transfer (FET) and vitrified embryo transfer (VET) were divided into two groups. Natural mating mice were used as the control. Introperitoneal glucose tolerance (IPGTT) and insulin tolerance test (IPITT) were performed in the adulthood offsprings. Total RNA was extracted from 14.5dpc fetus and neonate livers, and glycometabolism-related genes were detected by quantitative RT-PCR. Results: The glucose levels of IPGTT at 30 min and 120 min in offspring mice from VET were significantly higher than control, and the glucose level at 120 min was significantly higher than that in FET. In liver tissues from 14.5 dpc embryos, the mRNA expression of Igf1 and Igf2 in FET and VET groups, the mRNA expression of Glut8 in VET was significantly higher than that in the control. In livers of newly born mice, the mRNA expression of Igf1 and Igf2 in FETï¼ÂŒGlut1 and Glut2 in FET and VET was significantly lower than that in the control.VET and FET, especially VET, may affect the glycometabolism of offspring mice.

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