alexa Effectiveness of a Biological Insecticide Derived from Scorpion Fish Offal against the Chickpea Weevil
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Effectiveness of a Biological Insecticide Derived from Scorpion Fish Offal against the Chickpea Weevil

Laribi-Habchi H1, Biche M2, Drouiche N1,3*, Khalfi W2, Abdi N1 and Mameri N1
1National Polytechnic school of Algiers, B.P. 182-16200, El Harrach, Algiers, Algeria
2Institut National Agronomique d’Alger, El Harrach, Algeria
3Centre de Recherche en Technologie des Semi-conducteurs pour l’énergétique (CRTSE). 2, Bd Frantz Fanon BP140, Alger – 7 Merveilles, 16000, Algeria
Corresponding Author : Dr. Nadjib Drouiche
Technology of Semi-conductor for the Energetic Research Center
Department of Environmental Engineering
2, Bd Frantz Fanon BP140 Alger-7-merveilles
Algiers, Algeria
Tel: 21321279880 (Ext:192)
Fax: 21321433511
Email: [email protected]; [email protected]
Received Janaury 04, 2014; Accepted January 20, 2014; Published January 27, 2014
Citation: Laribi-Habchi H, Biche M, Drouiche N, Khalfi W, Abdi N, et al. (2014) Effectiveness of a Biological Insecticide Derived from Scorpion Fish Offal against the Chickpea Weevil. J Bioprocess Biotech 4:148 doi: 10.4172/2155-9821.1000148
Copyright: © 2014 Laribi-Habchi H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The aim of this study is to evaluate the biological activity of a crude chitinase extracted from fish offal (scorpion) on the chickpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae) at various concentrations: 3%, 6%, 9%, 12%, 15%, 18%, 21% and 24% (V/V). The biological tests were performed under the typical experimental conditions: temperature and relative humidity at 28°C and 75%, respectively. The results have shown that the concentration of 24% of crude chitinase extracted has 100% of insecticide activity after a contact time of 1 h. The lethal dose (LD50) is estimated for every sample and for a range time from 1 to 72 h. The lower percentage of mortality (10%) is obtained for low values of concentrations and contact time (dose of 3% of crude chitinase and 1 h of contact time). The maximum mortality rate is observed for the high values of two parameters (dose of 24% of crude chitinase and 72 h contact time). However, it is observed that for a higher dose (24%) and for a lower contact time (1 h), the mortality rate increased rapidly. The novelty of this work consists in the use of a new bioinsecticide obtained from offal scorpion fish.

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