alexa Effectiveness of Enzymatic Transesterification of Beeftallow Using Experimental Enzyme Ns88001 with Methanol and Hexane | OMICS International | Abstract
ISSN: 2329-6674

Enzyme Engineering
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Effectiveness of Enzymatic Transesterification of Beeftallow Using Experimental Enzyme Ns88001 with Methanol and Hexane

S Kumar, AE Ghaly*, MS Brooks, SM Budge and D Dave
Department of Process Engineering and Applied Science, Dalhousie University Halifax, Nova Scotia Canada
 
Corresponding Author : Abdel Ghaly
Professor, Department of Process Engineering and Applied Science
Faculty of Engineering, Dalhousie University
Halifax, Nova Scotia, Canada
Tel: (902) 494-6014
E-mail: [email protected]
 
Received November 13, 2013; Accepted November 28, 2013; Published December 06, 2013
 
Citation: Kumar S, Ghaly AE, Brooks MS, Budge SM, Dave D (2013) Effectiveness of Enzymatic Transesterification of Beeftallow Using Experimental Enzyme Ns88001 with Methanol and Hexane. Enz Eng 2:116. doi:
 
Copyright: © 2013 Kumar S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 
Visit for more related articles at
DownloadEnzyme Engineering

Abstract

 The effectiveness of enzymatic transesterification of animal fat using the experimental enzyme catalyst NS88001 was studied. The effects of oil: alcohol molar ratio (1:1, 1:2, 1:3, 1:4 and 1:5), reaction temperature (35, 40, 45 and 50°C) and reaction time (4, 8, 12 and 16 h) on the biodiesel conversion yield were evaluated. n-Hexane was used as a solvent. The highest conversion yield of biodiesel was obtained at the1:4 oil: alcohol molar ratio, 16 h reaction time and 45°C reaction temperature. The rate of conversion of fatty acid esters increased with increases in reaction time. The reaction proceeds slowly at the beginning due to the initial mixing and dispersion of alcohol into the oil and activation of enzyme. After dispersion of alcohol, the enzyme rapidly interacted with fatty acids esters. Increasing the reaction time from 4 to 16 h increased the biodiesel conversion yield by 13.05-71.94%, depending on the reaction

temperature and oil: alcohol molar ratio. Increasing the oil: alcohol molar ratio from 1:1 to 1:4 increased the biodiesel conversion yield by 32.68-82.11% while increasing the oil: alcohol molar ratio from 1:4 to 1:5 decreased the biodiesel conversion yield by 5.43-34.27%, depending on the reaction time and reaction temperature. The increase in the oil: alcohol molar ratio promotes the reaction between the enzyme and substrate. The interactions between enzyme polymer surface and substrate appears to be dependent on reaction temperature due to hydrogen bonding and ionic interactions which play a important roles in maintaining the thermostability of lipase in the system. Increasing the reaction temperature from 40 to 45°C increased the biodiesel conversion yield by 3.64-58.78%. Higher temperature from 45 to 50°C denatured the specific structure of enzymes and resulted in a decrease in biodiesel conversion yield by 4.3-32.47%. Using n-hexane in the reaction helped to stabilize the enzyme and minimize the toxicity of alcohol. The activity of experimental enzyme catalyst (NS88001) in the presence n-hexane was slightly reduced after 10 cycles. However, the enzyme activity decreased rapidly when the number of cycles was increased above 10 and stopped completely after 50 cycles.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7