alexa Effectiveness of the Androctonus Australis Hector Nanobody NbF12-10 Antivenom to Neutralize Significantly the Toxic Effect and Tissue Damage Provoked by Fraction of Androctonus mauretanicus (Morocco) Scorpion Venom
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
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Research Article

Effectiveness of the Androctonus Australis Hector Nanobody NbF12-10 Antivenom to Neutralize Significantly the Toxic Effect and Tissue Damage Provoked by Fraction of Androctonus mauretanicus (Morocco) Scorpion Venom

Chgoury F1,4*, Benabderrazek R2, Tounsi H3, Oukkache N1, Hmila I2, Boubaker S3, Ayeb ME2, Saïle R4 Ghalim N1 and Bouhaouala-Zahar B2,5*
1Laboratory of Venoms and Toxins, Institut Pasteur du Maroc, 1- Place Louis Pasteur, Casablanca 20360, Morocco
2Laboratory of Venoms and Therapeutic Molecules, Pasteur Institute of Tunis/University of Tunis El Manar, 13 Place Pasteur, BP74, 1002 - Tunis, Tunisia
3Laboratory of Human and Experimental Pathology, Pasteur Institute of Tunis/University of Tunis El Manar, 13 Place Pasteur, BP74, 1002 - Tunis, Tunisia
4Laboratory of Biology and Health, URAC 34, Hassan II University Casablanca, Faculty of Science Ben M’sik, Morocco
5Medical School of Tunis, 15 Rue Djebel Lakhdhar, La Rabta 1007, Tunis-Tunisia-University of Tunis El Manar
Corresponding Author : Fatima Chgoury
Laboratory of Venoms and Toxins
Institut Pasteur du Maroc, 1- Place Louis Pasteur
Casablanca 20360, Morocco Balkiss Bouhaouala-Zahar
Medical School of Tunis, 15 Rue Djebel Lakhdhar
La Rabta 1007, Tunis-Tunisia-University of Tunis El Manar
Tel: +212-662-472-795, +216-983-452-65
Fax: +212-522-260-957, +212-71-791-833
E-mail: [email protected], [email protected]
Received June 15, 2014; Accepted June 25, 2015; Published June 29, 2015
Citation: Chgoury F, Benabderrazek R, Tounsi H, Oukkache N, Hmila I, et al.(2015) Effectiveness of the Androctonus Australis Hector Nanobody Nbf12-10 Antivenom to Neutralize Significantly the Toxic Effect and Tissue Damage Provoked by Fraction of Androctonus mauretanicus (Morocco) Scorpion Venom. Biochem Pharmacol (Los Angel) 4:174. doi:10.4172/2167-0501.1000174
Copyright: © 2015 Chgoury F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Scorpion stings are life threatening in large parts of the world. Toxins from scorpion venom are responsible for severe metabolic and tissue disruption and immunotherapy is the only specific treatment able to neutralize the toxic effects of scorpion venom. The Androctonus mauretanicus (Am) is the most dangerous scorpion in Morocco, whereas in Tunisia Androctonus australis hector (Aah) is causing most casualties. In this work, we investigated the potential of NbF12-10, a new immunotherapeutic concept based on anti-toxin Nanobodies (Nbs) to neutralize Am scorpion venom. We first explored the immune cross-reactivity between Am and Aah scorpions venoms using anti-AahI and anti-AahII polyclonal, NbAahI’F12 (anti-AahI’), monospecific NbAahII10 (anti-AahII) and bispecific NbF12-10 (anti-AahI’/anti- AahII) monoclonal antibodies and subsequently we study the histological damages observed after envenomation with the F3 toxic fraction of Am scorpion venom by intra-cerebroventricular (i.c.v) injection and the capacity of NbF12-10 to reduce tissue damage induced by F3 fraction after i.c.v administration of F3:NbF12-10 mixture, in mice. Results showed significant para-specific activity of anti-Aah polyclonal and monoclonal antibodies towards Am venom fractions. Histological investigations revealed severe tissue damage in brain, lung and liver after i.c.v. administration of F3 fraction. The NbF12-10 pre-mixed with F3 fraction showed an efficient neutralizing capacity against lethal effect of this toxic fraction. Moreover, in vitro pre-incubation of F3 with NbF12-10 at 8-fold molar excess led to significantly reduced tissue damage. Further, NbF12-10 displays a noteworthy potential to neutralize Am toxins and to rescue 50% of envenomed mice from dying. This study provides first evidence that NbF12-10 nano-therapeutic has promising prospective to treat scorpion envenoming in the Maghreb area.

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