Effects of Antioxidants on the Viability of the Human Neuroblastoma SH-SY5Y Cell Culture under the Conditions of Heavy-Metal Toxicity
- Corresponding Author:
- Kulikova OI
Research Center of Neurology, 80
Volokolamskoe Shosse, Moscow 125367, Russia
E-mail: [email protected]
Received Date: April 27, 2016; Accepted Date: May 17, 2016; Published Date: May 25, 2016
Citation: Kulikova OI, Fedorova TN, Lopachev AV, Orlova VS, Grachev VA (2016) Effects of Antioxidants on the Viability of the Human Neuroblastoma SH-SY5Y Cell Culture under the Conditions of Heavy Metal Toxicity. Biol Med (Aligarh) 8:305. doi:10.4172/0974-8369.1000305
Copyright: © 2016 Kulikova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Protective action of antioxidants (mexidol, carnosine, N-acetyl cysteine) and the metal chelator Ca, Na2-EDTA was studied in the culture of human neuroblastoma SH-SY5Y cells after the addition of salts of heavy metals—lead, cadmium, cobalt, and molybdenum—to the culture medium. Cells were incubated with heavy metals and protectors for 24 h, and cell viability and cell death were evaluated. All the metals lowered cell viability in a concentration-related manner. Different protective agents were studied based on this model. The most pronounced capability of increasing the cell viability in conditions of heavy-metal toxicity was demonstrated by N-acetyl cysteine (the protective effect was demonstrated at the concentrations 0.5-1.0 mM and higher). Protective potential of carnosine was somewhat lower and that of mexidol was minimal.