Effects of Blood Collection Tubes on Determination Vitamin-A by HPLC
Sawsan Mohammed AH*, Azhar Abdul JB and Hammed A
Research Institute/Ministry of Health, Iraq
- *Corresponding Author:
- Sawsan Mohammed AH
Research Institute/ Ministry of Health, Iraq
E-mail: [email protected]
Received Date: May 03, 2013; Accepted Date: May 27, 2013; Published Date: June 02, 2013
Citation: Sawsan Mohammed AH, Azhar Abdul JB, Hammed A (2013) Effects of Blood Collection Tubes on Determination Vitamin-A by HPLC. J Chromat Separation Techniq 4:184. doi: 10.4172/2157-7064.1000184
Copyright: © 2013 Sawsan Mohammed AH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: VACUETTE® Blood Collection Tubes are used for the collection of venous blood. VACUETTE® tubes are used to collect, transport, store and process blood for testing serum, plasma or whole blood in the clinical laboratory for professional use.
Objective: Evaluate the effect of blood specimen collection methods of the VACUETTE Serum Sep Clot Activator tube (gel tube), in determination vitamin A by HPLC.
Methods: Blood specimen was divided into three parts, Three tubes, for Part I, No Additive tubes were drawn, for Part 2 Serum SepClot Activator tube (gel tube), and for Part 3 EDTA-coated plastic tubes. The tubes were gently mixed using ten complete inversions immediately following blood collection and centrifuged after venipuncture for 15 min and the supernatants were stored at -70°C until analysis. Vitamin A Testing was performed on healthy blood donors; the testing was performed by HPLC.
Results: Our study showed that, There was no difference in the results were obtained with the serum (No Additive tube) and plasma tube in concentration of retinol 67.7 ug/dl and 73.8 ug/dl, but the results was 9.2 ug/dl obtained with the VACUETTE® Serum Sep Clot Activator tubes (gel tube).
Conclusions: Tubes are drawn in a specific order to avoid the possibility of erroneous test results caused by carryover of an additive. If Serum tube-with or without clot activator or gel, it should be drawn as the first tube. We recommended that only serum and EDTA -plasma samples be used for Vitamin A determination by HPLC and cannot be carried out with all gel tubes.