Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-20-4587-4809
ISSN: 2167-0501
+44-20-4587-4809
The osmotic fragility (OF) of isolated Red Blood cells (RBCs) in guinea pigs and rats was examined by monocarboxylic, dicarboxylic and their partition coefficients. A phosphate–NaCl buffer solution that comprises each carboxylic acid at the concentration ranging from 0 to 100 mM was used to incubate dense-packed RBCs at 37°C for 60 minutes. The RBC suspensions were moved into OF test tubes containing NaCl from 0.1 to 0.8%. The hemoglobin concentration was determined and was observed that NaCl concentration induced 50% hemolysis as the OF value. The OF was was increased by dose dependently the exposure to some monocarboxylic acids that possessed hydrocarbon chains of more than 4 carbons in length in case of rats but no increase was observed in guinea pig RBCs. A positive correlation was obtained between the partition coefficients and the degree of change in OF for monocarboxylic acids in the rat, but not in guinea pig RBCs. On the other hand, dicarboxylic acids are corresponding to the monocarboxylic acids had either no effect or decreased OF in rat and guinea pig RBCs. There was no correlation between the partition coefficients and change in OF for monocarboxylic acids in only guinea pig RBCs but for dicarboxylic acids in both rat and guinea pig RBCs. The partition coefficients of the monocarboxylic acids were higher comparatively the corresponding dicarboxylic acids. Whereas monocarboxylic acids are thought to act on the hydrophobic acyl-chain of phospholipids in the deeper regions of the RBC membrane, dicarboxylic acids act on the hydrophilic interface and shallower areas of the RBC membrane. The fluidity index based on fatty acid composition was observed higher in the rat than the guinea pig RBC membrane. The difference in fatty acid composition was speculated to be one of the causes of different OF response to monocarboxylic acids between rat and guinea pig RBCs.