Effects of Conjugated Linoleic Acid and Hilsha Fish Oil (Tenualosa Ilisha) on Alloxan Induced Diabetes Mellitus in Sprague Dawley RatsIslam Minarul1, Manirujjaman M1, Khan MMH1, Rahman Matiar1, Amin M Ziaul2, Uddin AMM1, Biswas Shahangir1 and Islam MA1*
- *Corresponding Author:
- Mohammad Amirul Islam Ph.D
M.Sc. (Biochemistry), Professor
Department of Biochemistry and Molecular Biology
University of Rajshahi, Rajshahi-6205, Bangladesh
Tel: + 880-171-2141273
Fax: + 880-721-750064
E-mail: [email protected]
Received date: May 13, 2014; Accepted date: July 27, 2014; Published date: August 18, 2014
Citation: Minarul I, Manirujjaman M, Khan MMH, Matiar R, Ziaul AM, et al. (2014) Effects of Conjugated Linoleic Acid and Hilsha Fish Oil (Tenualosa Ilisha) on Alloxan Induced Diabetes Mellitus in Sprague Dawley Rats. J Diabetes Metab 5:412 doi: 10.4172/2155-6156.1000412
Copyright: © 2014 Minarul I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Diabetes mellitus is a multifarious disease. There is no specific cause for the development of this disease. Fish oil plays important role for the remediation of diabetes mellitus. Conjugated Linoleic Acid (CLA) was synthesized by five steps that included saponification of soybean oil, low temperature crystallization of saturated fatty acid, urea crystallization of oleic acid, alkali isomerization of linoleic acid to CLA and fatty acid analysis. Fish oil was extracted from sun dried fish flesh by n-hexane. The rats were divided into five groups. The Group-1 and Group-2 considered as normal and Diabetic Control. Group-3, Group-4 and Group-5 diabetic rats were treated with CLA, Fish oil and Glibenclamide respectively. Diabetes Mellitus (DM) was induced by single intra peritoneal injection of freshly prepared alloxan hydrate (55 mg/kg Body Weight of rat) in 0.9% saline solution. The significant effects (P<0.001) of CLA and fish oil were observed in alloxan-induced diabetic rats to control the blood glucose level within range. Antilipidemic effects of CLA and fish oil were also observed and found to reduce the cholesterol and LDL level whereas increased the HDL level significantly (P<0.05). Like glibenclamide CLA and fish oil had significant effects on the control of alloxan induced diabetes mellitus in Sprague Dawley rats.