alexa Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis
ISSN: 2329-6674

Enzyme Engineering
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Research Article

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

Naznin Akhtar1*, Abu Sayeed Mohammad Mahmud2, Muhammad Shahjalal Khan3, Tarannum Taznin4, Muhammad Ehteshamul Haque4, Sharmin Sultana4 and Sharmin Sultana4
1Tissue Banking and Biomaterial Research Unit (TBBRU), Atomic Energy Research Establishment (AERE), Savar, Dhaka, Bangladesh
2Industrial Microbiology Research Division, BCSIR Laboratories, Bangladesh
3Department of Microbiology, University of Chittagong, Bangladesh
4Department of Microbiology, Jessore Science and Technology University, Bangladesh
Corresponding Author : Naznin Akhtar
Scientific Officer
Tissue Banking and Biomaterial Research Unit (TBBRU)
Atomic Energy Research Establishment (AERE)
Savar, Dhaka, Bangladesh
E-mail: [email protected]
Received February 05, 2013; Accepted February 10, 2013; Published February 20, 2013
Citation: Akhtar N, Mahmud ASM, Khan MS, Taznin T, Haque ME, et al. (2013) Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis. Enz Eng 2:110. doi:10.4172/2329-6674.1000110
Copyright: © 2013 Akhtar N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Proteolytic activity of two isolates of actinomycetes, Streptomyces albolongus and Streptomyces aburaviences was investigated on the basis of their ability to hydrolyze skimmed milk casein, egg albumin and gelatin. Both isolates were found to have potential for extracellular proteases production. Effects of culture conditions for the production of extracellular protease from S. albolongus and S. aburaviensis were determined. Highest protease yield from S. albolongus was obtained after 5 days of incubation with an initial pH 7, at static state when inoculated in medium composed of 1% glucose, 2% beef extract, 0.2% yeast extract, 0.1% KH2PO4, 0.3% K2HPO4, and trace MgSO4.7H2O. Optimum incubation conditions for S. abureviences were 4 days, with an initial medium pH 8 at shaking condition (100 rpm). S. aburaviences preferred 1.5% lactose and 1.5% tryptone as a carbon and nitrogen source. Both the isolates showed maximum protease yield at 37°C. The result of the present study might be helpful for largescale production of extracellular protease from these Streptomyces spp.

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