Pharmaceutical Analytical Chemistry: Open Access
Open Access

Abstract

Effects of Ethanol Extract and its Different Fractions of Phrynium imbricatum(Roxb.) Leaves on In Vitro Anthelmintic and their Condensed Tannin Content

Mohammad Shah Hafez Kabir, Abu Monsur Dinar Md, Mohammed Munawar Hossain, Muhammad Abdulla Al Noman, Fahima Zaheed, Rabiul Hossain Md, Abul Hasanat, Raju Dash, Mominur Rahman Md, and Zahid Hosen SM

Background: To identify the therapeutic effects of ethanol extract and its different fractions of Phrynium imbricatum leaves in anthelmintic (in vitro) and to determine their total condensed tannin content.

Methods: Leaves of Phrynium imbricatum was extracted with pure ethanol and fractioned with chloroform (CHFPI), petroleum ether (PEFPI), n-hexane (NHFPI) and ethyl acetate (EAFPI), which are tested for anthelmintic activity on aquarium worm Tubifex tubifex by using three concentrations viz., 5, 10 and 20 mg/ml of each. Total condensed tannin content determined based on the procedure of Oyedemi et al.

Results: Among the various crude extract, ethanol extract Phrynium imbricatum (EEPI) exhibited strong anthelmintic activity in vitro. Where it paralyzed (3.69 ± 0.25 min; P<0.001) and produced death (14.28 ± 0.44 min; P<0.001) of the Tubifex tubifex at 20 mg/ml dose near the value of the standard, Levamisole (3.3 ± 0.38 min and 6.5 ± 0.76 min) at 1 mg/ml. The content of condensed tannin good at EEPI and its all fractions, but EEPI (168.44 ± 0.87 mg catechin/g) contained highest among them. For both of experiment, activity found as follows, EEPI>CHFPI>EAFPI>NHFPI>PEFPI.

Conclusion: These findings suggest that the plant may be a potential source for the development of new anthelmintic and condensed tannin may one of such phytochemical, which exhibit anthelmintic activity