Effects of Ethanol Extract and its Different Fractions of Phrynium imbricatum(Roxb.) Leaves on In Vitro Anthelmintic and their Condensed Tannin Content
Mohammad Shah Hafez Kabir1, Abu Monsur Dinar Md2, Mohammed Munawar Hossain1, Muhammad Abdulla Al Noman3, Fahima Zaheed3, Rabiul Hossain Md3, Abul Hasanat1, Raju Dash4, Mominur Rahman Md1, and Zahid Hosen SM5*
- *Corresponding Author:
- Zahid Hosen SM
Pharmacology Research Division
Bangladesh Council of Scientific and Industrial Research (BCSIR)
E-mail: [email protected]
Received date: November 28, 2015; Accepted date: December 17, 2015; Published date: December 19, 2015
Citation: Kabir MSH, Md AMD, Hossain MM, Noman MAA, Zaheed F, et al. (2015) Effects of Ethanol Extract and its Different Fractions of Phrynium imbricatum (Roxb.) Leaves on In Vitro Anthelmintic and their Condensed Tannin Content. Pharm Anal Chem Open Access 1:109. doi: 10.4172/2471-2698.1000109
Copyright: © 2015 Kabir MSH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: To identify the therapeutic effects of ethanol extract and its different fractions of Phrynium imbricatum leaves in anthelmintic (in vitro) and to determine their total condensed tannin content.
Methods: Leaves of Phrynium imbricatum was extracted with pure ethanol and fractioned with chloroform (CHFPI), petroleum ether (PEFPI), n-hexane (NHFPI) and ethyl acetate (EAFPI), which are tested for anthelmintic activity on aquarium worm Tubifex tubifex by using three concentrations viz., 5, 10 and 20 mg/ml of each. Total condensed tannin content determined based on the procedure of Oyedemi et al.
Results: Among the various crude extract, ethanol extract Phrynium imbricatum (EEPI) exhibited strong anthelmintic activity in vitro. Where it paralyzed (3.69 ± 0.25 min; P<0.001) and produced death (14.28 ± 0.44 min; P<0.001) of the Tubifex tubifex at 20 mg/ml dose near the value of the standard, Levamisole (3.3 ± 0.38 min and 6.5 ± 0.76 min) at 1 mg/ml. The content of condensed tannin good at EEPI and its all fractions, but EEPI (168.44 ± 0.87 mg catechin/g) contained highest among them. For both of experiment, activity found as follows, EEPI>CHFPI>EAFPI>NHFPI>PEFPI.
Conclusion: These findings suggest that the plant may be a potential source for the development of new anthelmintic and condensed tannin may one of such phytochemical, which exhibit anthelmintic activity