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Effects of Exposure to Plasticizers Di-(2-Ethylhexyl) Phthalate and Trioctyltrimellitate on the Histological Structure of Adult Male Albino Ratsí Liver | Abstract
ISSN: 2161-0495

Journal of Clinical Toxicology
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Research Article

Effects of Exposure to Plasticizers Di-(2-Ethylhexyl) Phthalate and Trioctyltrimellitate on the Histological Structure of Adult Male Albino Rats’ Liver

Azza Saad Shehata, Zeinab Abd El-Rehim Mohamed*, Manal Reda Abd El-Haleem and Mai Amin Samak

Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Egypt

*Corresponding Author:
Zeinab Abd El-Rehim Mohamed
Histology and Cell Biology Department
Faculty of Medicine, Zagazig University, Egypt
E-mail: [email protected]

Received date: June 19, 2013; Accepted date: September 26, 2013; Published date: October 11, 2013

Citation: Shehata AS, El-Rehim Mohamed ZA, El-Haleem MRA, Samak MA (2013) Effects of Exposure to Plasticizers Di-(2-Ethylhexyl) Phthalate and Trioctyltrimellitate on the Histological Structure of Adult Male Albino Rats’ Liver. J Clin Toxicol 3:169. doi: 10.4172/2161-0495.1000169

Copyright: © 2013 Shehata AS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Di (2-ethylhexyl) phthalate (DEHP) and Trioctyltrimellitate (TOTM) are plasticizers used to increase the flexibility of polyvinyl chloride. Their wide commercial usage carries high risk of human exposure. This work aimed to investigate histological changes of rat’s liver upon DEHP and TOTM exposure. 36 adult male albino rats were classified into 3 equal groups; control group, group II (given DEHP 300 mg/kg) and Group III (given TOTM 300 mg/ kg) orally for 4 weeks. Half of the animals were sacrificed after one day of last dose (subgroups IIA & IIIA) and the remaining were left for another 4 weeks for recovery (subgroups IIB & IIIB). Liver specimens were examined by light and electron microscopes and immunohistochemical stain for hepatocyte paraffin -1 (Hep Par-1). By examination of subgroup IIA all rats’ liver showed focal changes. Dramatically affected lobules revealed loss of lobular architecture with periportal cellular and fatty infiltrations. Hepatocytes had shrunken nuclei, peroxisomes and lipid globules. Subgroup IIB showed mild morphological improvement. Subgroup IIIA showed preserved lobular architecture with vascular congestion. Hepatocytes showed euchromatic nuclei and lipid globules. Subgroup IIIB revealed apparent normal lobular architecture and normal hepatocytes. Comparing to control group, the area % of Hep Par-1 immune reaction in subgroup IIA was highly significant decrease while group IIB was significantly decreased. Subgroup IIIA was significantly decreased. However, group IIIB had no significant difference with control. In conclusion, DEHP induced serious changes on liver; most of these changes were irreversible. TOTM has mild reversible effects. It is recommended to use safely as an alternative plasticizer.


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