Effects of Four Herbal Plants on Kidney Histomorphology in STZ-induced Diabetic Wistar Rats
- *Corresponding Author:
- Komolafe OA
Department of Anatomy and Cell Biology
Faculty of Basic Medical Sciences
Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
E-mail: [email protected]
Received Date: October 30, 2013; Accepted Date: December 26, 2013; Published Date: December 28, 2013
Citation: Komolafe OA, Ofusori DA, Adewole OS, Ajayi SA, Ijomone MM, et al. (2013) Effects of Four Herbal Plants on Kidney Histomorphology in STZ-induced Diabetic Wistar Rats. J Cytol Histol 5:210. doi:10.4172/2157-7099.1000210
Copyright: © 2013 Komolafe OA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The aim of this study is to investigate the effect of four herbal extract and their efficacy on the histomorphometry of the kidney in STZ induced diabetic rats with a view to understanding their antidiabetic properties. Forty- two healthy adult Wistar rats (Rattus novergicus) with an average weight of 153.4 g were randomly divided into seven groups (n=6). STZ (65 mg/kg) dissolved in citrate buffer was administered intraperitoneally to animals in groups (B-G) while animals in group A received equivalent volume of citrate buffer. Plant extracts (100 mg/kg) were administered daily (orally) to animals in groups C-F and glimepiride (anti-diabetic drug) to animals in group G for fourteen days. After the expiration of the study the animals were sacrificed and the kidneys were excised, fixed in 10% formol saline for histology and morphometric analysis. The glomeruli of the diabetic group were atrophied which is validated by significant decrease in its density, shrinkage and increased bowman’s space. These observations were also characterized by diminished cellular proliferation, decreased cellular volume and ischemia. The histology and morphometric analysis revealed that the kidney in the group treated with Psidium guajava shows a better histoarchitectural outline of all the four plant extracts used. This present study therefore suggests that Psidium guajava could be a better alternative therapy in ameliorating diabetic-associated disorders of the kidney.