Effects of IgM Anti-Galnac-GD1a Monoclonal Antibody on Neuromuscular Transmission and Calcium Channel Binding in the Rat Neuromuscular JunctionSayako Hotta1, Takumi Nagaoka1, Yoshihiko Nakatani1, Toshie Kambe2, Kenji Abe3, Yutaka Masuda4, Iku Utsunomiya5 and Kyoji Taguchi1*
- Corresponding Author:
- Kyoji Taguchi
Department of Medicinal Pharmacology
Showa Pharmaceutical University
Machida, Tokyo 194-8543, Japan
E-mail: [email protected]
Received date: January 05, 2015; Accepted date: March 16, 2015; Published date: March 23, 2015
Citation: Hotta S, Nagaoka T, Nakatani Y, Kambe T, Abe K, et al. (2015) Effects of IgM Anti-Galnac-GD1a Monoclonal Antibody on Neuromuscular Transmission and Calcium Channel Binding in the Rat Neuromuscular Junction. J Neurol Neurophysiol 6:275. doi:10.4172/2155-9562.1000275
Copyright: © 2015 Hotta S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Guillain-Barré syndrome with antibodies against ganglioside N-acetylgalactosaminyl GD1a (GalNAc-GD1a) is
characterized by a rapid onset of predominantly distal pure motor neuropathy. However, the pathogenic role of anti-
GalNAc-GD1a antibody and calcium channels in neuromuscular junctions (NMJs) remains unclear. We investigated
the effects of IgM anti-GalNAc-GD1a monoclonal antibody (IgM anti-GalNAc-GD1a mAb) on spontaneous muscle
action potentials (SMAP) in a rat spinal cord–muscle co-culture system and the localization of IgM anti-GalNAc-
GD1a mAb and calcium channel binding in the rat hemi-diaphragm. Immunohistochemistry of the rat hemidiaphragm
showed that IgM anti-GalNAc-GD1a mAb binding overlapped with anti-neurofilament 200 antibody and α-
bungarotoxin staining, demonstrating that IgM anti-GalNAc-GD1a mAb was localized at the motor nerve terminal.
Moreover, IgM anti-GalNAc-GD1a mAb binding overlapped with anti-Cav2.1 antibody in the nerve terminal. We
suggest that the inhibitory effect of IgM anti-GalNAc-GD1a mAb on SMAP is related to the GalNAc-GD1a epitope on
P/Q-type calcium channels in motor nerve terminals at NMJs.